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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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Introduction: This experiment was used to examine the hypothesis that: Osmosis is dependent on the concentrations of the substances involved. Diffusion is the passage of solute molecules from an area of high concentration to an area of low concentration (Campbell & Reece‚ 2005). An example is ammonia diffusing throughout a room. A solute is one of two components in a chemical solution. The solute is the substance dissolved in the solution. The solvent‚ the other component‚ is any liquid in
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The Effect of substrate on the Rate of Respiration on Yeast1 Justine Maturan Group 4 Sec. Y – 5L November 18‚ 2014 ________________________________________________________________ 1A scientific paper submitted in partial fulfilment of the requirements in General Biology I Laboratory under Prof. Susan Sedano‚ 1st semester 2014-2015 ABSTRACT In order to determine the effect of the substrate on the rate of respiration of yeast‚ Durham test tube method was used in the first experiment
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Isolation and Characterization of Maize Acid Phosphatase | BCHM 405 | Aaron BANESEH 10307275 | Table of Contents 1 Table of Contents 2 2 Introduction 4 3 Experimental Procedures 5 3.1 Sample preparation 5 3.2 Initial Extraction of Acid Phosphatase 5 3.3 Determination of Protein Concentration by the Folin-Lowry Assay 5 3.4 Specific Activity Assay 6 3.4.1 Definition of Enzyme Unit and Specific Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of
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to accept and unshared pair of electrons from a base. An alkaline is a chemical compound that neutralizes or effervesces with acids and turns litmus blue; it’s a corrosive substance. Two similarities between acid and alkaline is that they are sour and they each changes litmus paper into another color than it was originally was. The differences between an acid and an alkaline are that acids have a Ph scale of any numbers below 7 and alkaline is from any numbers that is 7 or greater. I have searched
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Title of Practical: The Effect of Concentration on Reaction Rate Aim: To use a simple reaction between sodium thiosulphate and hydrochloric acid to discover concentration this determines how fast chemical reactions occur. Independent Variable: Concentration of hydrochloric acid (%) Dependent Variable: Time taken for chemical reaction to take place (sec) Hypothesis: My prediction is that the increased concentration of the thiosulfate will in turn lead to an increase in the rate of reaction
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regulate other physiological processes‚ as well as being responsible for the activation of some ion channels and enzymes and assisting in trafficking of proteins to the cell membrane (5‚ 8). Why is PIP2 then so important in hippocampal cells? PIP2 is a substrate for hydrolysis by the enzyme phospholipase C (PLC)‚ whereby the products of this interaction are the secondary messengers‚ diacyl Glycerol (DAG) and inositol trisphosphate (IP3) (6‚ 8) that are involved in the regulation of physiological processes
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OBJECTIVE: The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different
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Abstract The following experiment details the effect of different concentrations of catalase on the production of oxygen and water through the breakdown of Hydrogen Peroxide. In this experiment paper disc where coated in varying concentrations of catalase‚ 0‚ 25‚ 50 75 and 100%. The time taken for the disc to float between two markers on the side of a glass was then recorded. This experiment demonstrates that the higher the concentration of enzyme used the greater the production of oxygen on the
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