Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. DNA is a polymer‚ which is a chemical compound or a mixture of compounds consisting of repeating structural units. These repeating structures are created through polymerization. The monomer‚ meaning one part‚ units of DNA are nucleotides. Each nucleotide consists of a five carbon sugar‚ also known as deoxyribose‚ and nitrogen containing base attached to the sugar
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chromosomes are very long compact coils of DNA (Deoxyribonucleic Acid) that store all the information that the body inbeds such as how one looks and functions. This paper will first describe the structure of DNA; second discuss how the structure of DNA allows it to serve as the basis for inheritance‚ third examine how meiosis allows DNA to be divided into gametes and finally‚ describe how this relates to Gregor Mendel’s patterns of inheritance. The structure of DNA DNA is a thread formed by two strands
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advances in harvesting mitochondrial DNA. “Markers” are used to trace ancestry. These markers are found through DNA Sequencing and SNP testing. The general acceptance is that the human race stemmed from a woman referred to as “Mitochondrial Eve.” It is estimated that she lived 200‚00 years ago in Africa (Rice University). Margit M. K. Nass and Sylvan Nass are accredited in discovering Mitochondrial DNA‚ or mtDNA in 1960 using electron microscopy (Rice University). DNA is found in two places in the cell;
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ions -DNA replication minimizes errors such as the DNA that is duplicated but changes in the DNA do occur‚ producing mutations. Although most mutations are either neutral or harmful they are also the raw material for evolution. Such mutations from alleles‚ alternate forms of a given gene that may produce differences in structure or function such as black‚ brown or blond hair in humans‚ or different mating calls in frogs. Stages of Mitosis~ 1)Parent cell. 2)Chromosomes make identical copies
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to that person‚ therefore it is a great resource for police to help locate people involved in a case. Families share many of the same traits in their DNA but people are unsure of whether or not they have similarities‚ In this experiment that question will be answered. There are three main fingerprint types: arches‚loops‚and whorls. (GeneEd - DNA Fingerprints 2003‚ April 12). The police take fingerprint samples from the crime scene or from involved people). Once the fingerprints are taken and
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DNA EXTRACTION In extracting chromatin from the cells of wheat germ there are seven steps to follow. The optimal cell to use would be the polyploidal eukaryotic. Eukaryotes have nucleus membrane-bound organelles‚ while prokaryotic does not. The polyploidal eukaryotic cell has DNA that is held in the nucleus while the prokaryote has DNA that floats freely around the cell. The DNA of eukaryotes is more complex and extensive than the other. Prokaryote is a bacterial cell that
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DNA Worksheet Misanna Gordon SCI 230 December 02‚ 2012 Mitzie Sowell Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. A DNA molecule which is abbreviation for deoxyribonucleic acid is made up of very long chains of monomers and polymers that are called nucleotides. These two chains in particular which composes of DNA strain are then formed by the grouping of the nucleotides into the polynucleotides. The
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will finish off talking about PCR‚ and then we will discuss how it can be used. If we go back to the slide of the double stranded DNA‚ and if we take that to a high temperature‚ the two strands separate‚ you then add the primers‚ which interact with ? On the strand‚ synthesis takes place in the 5-3 direction‚ then you end up with 2 molecules identical to the DNA‚ and then you do another round‚ so it’s an exponential increase. There are different enzymes and polymerases‚ which come from bacteria
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life (1). Significant developments have been made in gene monitoring techniques specifically in DNA microarrays which only very recently revolutionized genome expression analysis (1). Despite continuous improvements and modification to the technique‚ DNA microarrays are still no more than a glass microscope slide studded with individual immobile nucleotide fragments (1‚ 2). The fundamentals of DNA microarrays are set on complementary base-pairing (3)‚ and because the exact sequence and position
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