Lab Report The effect of temperature on the reaction between Catalase and H2O2 Sarah AlShemesi In this experiment we’ll be exploring the effects of temperature on the reaction between Catalase and H2O2.We’ll be using five different temperatures to test this. The five different temperatures will be 10‚ 30‚ 50‚ 70 and 90 oC. We will use the liver as a source of Catalase. A 1 gram piece of liver will be inserted into a test tube with 2 cm3 of water‚ then 2 cm3 of H2O2 will be added. The Catalase
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Research Question Does the concentration of substrate (H2O2 (hydrogen peroxide)) have an effect on the activity of the enzyme (catalase)? Theory The higher the substrate concentration the more quickly product is produced (rate of reaction increases) until enzyme saturation is reached at which time more substrate has no further effect. Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all
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An experiment to investigate the effect of temperature on the rate of reaction of the Enzyme Trypsin. Aim: This investigation was on the effect temperature has on the rate that the enzyme trypsin hydrolyses its substrate‚ a protein found in milk (casein). This investigation was conducted under controlled conditions‚ the temperature being the changeable variable. Trypsin and its substrate (powdered milk which is a source of the protein casein) were heated in a water bath. The contents of the two
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Introduction In this experiment I am going to investigate the effect of varying concentration of a differing glucose solution on the amount of osmotic activity‚ between the solution and a potato tuber of a given size. The purpose of this experiment is to demonstrate how living cells rely on osmosis‚ the diffusion of water. Osmosis is the movement of water molecules (H20) from a region in which they are highly concentrated to a region in which
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The Effect of Concentration Gradient on Osmosis Abstract: Osmosis is the passive movement of water from an area of higher concentration to an area of lower concentration‚ usually across a membrane (Thorpe 2013). Tonicity is the ability of a solution surrounding a cell to gain or lose water (Reece 2011). There are many factors that affect the rate of osmosis. These include temperature‚ surface area‚ difference in water potential‚ pressure‚ light and dark and most importantly what we will be talking
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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changes in enzyme function? Objective The objective of this experiment is to observe if changes in temperature will cause a disruption in enzyme function. Hypothesis If we add and boil enzyme in L-Dopa‚ then the color will become darker because the temperature will denature some of the enzymes. Variables Independent: Temperature Dependent: Color change Control: Time‚ Room temperature Jobs We were assigned different jobs to perform the experiment Janelee had two jobs because we were
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I am choosing to study how the PSI (pounds per square inch) of a football is affected by the temperature. I chose to study this because I like football and I always pondered this question. I believe that this is a worthwhile question to study because these studies can help improve the game as the weather gets colder. As air cools‚ it expands‚ so when a football gets colder‚ the air inside it expands. This expansion causes the air pressure to decrease. The air pressure decreases because the molecules
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the beetroot cells. Osmosis‚ which is the net movement of water particles from a region of higher water potential to a region of lower water potential through a selectively-permeable membrane until equilibrium is reached‚ occurs the most for the beetroot cells which are submerged in the 4ml of 50% alcohol. This is because‚ the 4ml of 50% alcohol is the most concentrated solution among the 5 set-ups‚ hence would be the most hypertonic solution to the beetroot cells. Hence‚ osmosis would occur more rapidly
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Lab Report: How Temperature affects Reaction Rate Aim: The Aim is to investigate how temperature can affect Reaction Rate. The experiment will be performed by heating equally sized and weighted lime stones with equal amounts and concentration of Hydrochloric acid at different temperatures. The temperatures will be 35˚C and 40˚C. We will measure the reaction rates by observing gas release of the reaction between lime stones and Hydrochloric acid. The amount of gas release at different
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