Background Information Part 1 In the first part of the enzyme lab‚ we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together‚ we observed and saw a change in the color of the substance. The darker and more brown the substance got‚ the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased
Premium Enzyme PH
Lab Report: Enzyme Lab Your Name: Samantha Butcher Purpose of this Lab What is the goal of this lab? What question is it trying to answer‚ or what problem is it trying to explain? It is going to explain how a liver’s enzymes break down substances that could be harmful.| Hypothesis After reading the lab instructions - but before starting the lab - record your best “educated guess” about what will happen in the experiment. Give your reasons and outline any assumptions that lead
Premium Oxygen Gas Hydrogen peroxide
Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat is a
Premium
Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
Premium DNA Gene Molecular biology
Enzymes are proteins that increase or decrease the rate of chemical reactions. They are generally globular proteins and are around 62 amino acids residues in size. What enzymes do is determined by their 2-dimensional shape. A lot of enzymes are bigger than the substrate they act on‚ but only a little part of the enzyme involved directly with the catalysis. Without enzymes the chemical reactions in the body‚ would be so slow‚ the body would shut down. And cell reactions would take too much energy
Premium Enzyme Catalysis Chemical reaction
How Temperature Affects Amylase Activity Abstract This study of the effect of temperature on the enzyme amylase was performed to determine the relationship between the enzyme amylase and temperature. The rate of reaction was found to increase as the temperature of the environment was raised. As the temperature was raised from 5°C‚ 20°C‚ 35°C and finally to 80°C the rate of reaction followed this trend and also increased. However as predicted in the hypothesis of this experiment when the temperature
Premium Enzyme Chemical reaction Temperature
HINTS ON WRITING YOUR REPORT Title: Enzyme activity Objective: To investigate the effect of temperature on amylase activity Design principle Background: Amylase activity products? (show the equation) Which factors will affect enzyme activity? How to study the rate of reaction? (e.g. rate of disappearance of substrates or rate of formation of products) Independent variable: temperature of reaction mixture or at which the enzymatic reaction occurs. It can be varied by setting water bath at
Free Enzyme Starch
Ye Tao BISC220-13155 The Effect of Temperature on the Digestion of Starch by Activity of Enzyme α-Amylase: Observation of Rate of Starch Disappearance through Iodine Test Introduction An enzyme is a type of protein that‚ through its own structure including hydrogen bonds‚ acts like a biological catalyst and is able to accelerate the biochemical reaction rate by lowering the activation energy of the whole process‚ without which cells could hardly practice any physiological functions within human
Free Enzyme Starch
Introduction Enzymes are protein based structures that help speed up chemical reactions. They help these reactions keep up with the everyday metabolic needs and other like functions of organisms. Enzymes are also considered catalysts‚ due to the lowering in activation energy‚ in which they are not consumed or changed at any point during the reaction. These enzymes have three main protein structures that help keep them formed and intact. Stage 1 of these structures is the primary structure‚ which
Premium Enzyme Chemical reaction Metabolism
The Determination of the Effect of pH on Amylase Activity Grace Chung Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes‚ including amylase‚ function best at a certain optimal pH. Therefore‚ in this experiment‚ the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to
Premium Enzyme Starch Amylase