Effect of Temperature on Peroxidase Ability to Break Down H2O2 By: Rodneika Crutcher Abstract Temperature affects the ability of peroxidase to break down hydrogen peroxide. In this experiment our professor extracted peroxidase from potato tissue. In order to determine how temperature affects peroxidase we created solutions and measured their absorbance levels after water bath treatments. The more absorbent the solution was the less hydrogen peroxide there was in the solution. This means the peroxidase
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Publication No. 11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity
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you vary the temperature‚ after a certain temperature‚ the enzymes will become denatured and then the rate will decrease. Each enzyme functions at an optimum pH. Well according to the graph‚ you see that the enzyme did not function at all when boiled which can be explained by the fact that it got denatured. Denaturing means the secondary and tertiary structure of the enzyme does get disrupted so bonds would definitely be broken. What seemed to be the optimum temperature and pH for the
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Four environmental factors of enzymes were tested in lab. The changing of pH‚ substrate concentrations‚ temperature‚ and an inhibitor (NaCl) and the effects it hade on the enzyme turnip peroxidase. Enzymes are biological catalysts which increase reaction rates by lowering the activation energies of substrates. A substrate is a reactant that interacts with the enzyme. The enzyme and substrate can be viewed as the recently discovered "induced fit model"‚ which suggests enzymes are flexible and dynamic
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TITLE Analyzing the Effects of pH‚ Temperature‚ and Concentration on Peroxidase ABSTRACT The purpose of this experiment was to inspect the effect of environmental conditions on hydrogen peroxidase. The three conditions tested were the effect of peroxidase concentration on the rate of the experiment‚ the effect of pH of the rate of peroxidase activity‚ and the effect of temperature on the rate of peroxidase activity. During the lab‚ the lab group tested 7 test tubes‚ including 1 blank‚ with different
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Effects of Temperature‚ PH‚ boiling and concentration on Horseradish Peroxidase ABSTRACT The purpose of this report is to find out the effect of change in the Temperature‚ PH‚ boiling‚ concentration in peroxidase activity. Peroxidase is an enzyme that converts toxic hydrogen peroxide (H2O2) into water and another harmless compound. In this experiment we use‚ turnips and horseradish roots which are rich in the peroxidase to study the activity of this enzyme. The activity of peroxidase with change
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laboratory‚ we experimented and tested the reaction rate of a peroxidase enzyme and the factors that affected it‚ both positively and negatively. The purpose of these experiments was to probe and manipulate the activity of the enzyme peroxidase by varying temperature‚ pH‚ the amount of enzyme compared to the substrate and the effect of hydroxylamine. Peroxidase activity is expressed when the potato extract is subjected to stresses such as low temperature (El-hilali et al.‚ 2012). The most eye catching factors
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Abstract The enzyme peroxidase has been shown to break down H2O2. Enzymes are known to increase the rate at which a chemical reaction occurs. We looked at factors that affected the breakdown of hydrogen peroxide. These effects are the different temperatures and pH levels the enzymes were placed in. We found that the optimum‚ or best condition‚ temperature for the enzymes tested was about 22 degrees Celsius. The optimum pH level for the enzyme was 7. Introduction Enzymes are biochemical that catalyze
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December 2012 1. Title and Author 1. The Effect of Extreme Temperatures on the Rate of Photosynthesis 2. Jeffrey Xia 2. Abstract A previous lab in which we conducted‚ tested whether or not the light intensity had an effect on the rate of photosynthesis. We concluded that light intensity did possess an effect on the rate of photosynthesis – the closer the experimental plant units were to the light source‚ the more experimental plant units exhibited the effect of gas exchange in photosynthesis.
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process can be affected by several variables; one of the most notable variables is the temperature of the plant’s environment. Previous study has led me to conclude that when the temperature of the plants’ environment is low‚ the rate of photosynthesis is also low because the enzymes responsible for photosynthesis are not able to absorb as much heat energy and in turn will move slower. In contrast‚ if the temperature of the plant’s environment increases‚ the rate of photosynthesis will also increase
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