create a DNA profile in this experiment were PCR and gel electrophoresis. The PCR is used to amplify the several DNA samples and gel electrophoresis is performed to separate the DNA fragments according to their size. [6] In the first part of the experiment‚ PCR amplification of the DNA templates was performed and the products obtained were used to perform gel electrophoresis. The process of PCR allows for the amplification of the DNA samples and the components needed to perform PCR are template DNA‚ DNA
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We placed the lambda DNA into three test tubes. We then incubated one sample with EcoRI‚ one with HindIII‚ and the other we left as a control. After we completed the experiment and ran the DNA‚ we found that the first band of the crime scene DNA traveled 3.5mm and the second band traveled 6.5mm. The actual size of the first crime scene band is 1‚100bp and the second band is about 5‚500bp. The first band of suspect 1 DNA traveled 3 mm and the second band traveled 6.5mm
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bactaria was isolated and cloned. The expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E. coli. The ligation gave 15 colonies of recombinant DNA which later gave 6 recombinant plasmids as revealed by gel electrophoresis. 1. Introduction The NADH-dependent lactate dehydrogenase (EDH) is a key enzyme in the fermentative metabolism
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BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
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The universal DNA database is important to the law enforcement agencies. The database will help the law enforcement agencies to identify suspects fast. When the universal data base is not used‚ it is very easy for a criminal to commit a crime and escape. The DNA universal database will improve the crime investigation thereby curbing the menace of criminals. When this takes effect‚ criminals might be extremely careful not to leave any trace that can help in getting their DNA for identification (Krimsky
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It is when a bacterial cell takes up DNA that is foreign to it and integrates it into its own DNA. What is genetic transformation? That is change that is caused by genes involving the insertion of a gene into an organism to change the organism trait. This transformation typically occurs in plasmids. So‚ what are plasmids? Plasmids are small circular DNA molecules that are isolated from its chromosomes. So‚ what are genes and plasmids and how are
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ABSTRACT Genetics is a trend these days especially that‚ DNA barcoding has been developed. DNA barcoding is an important tool in categorizing the taxa of different species and it tells so much about the species’ traits‚ including genetic diversity. The Pomacea canaliculata was introduced in different parts of Asia and had been an invasive species and a pest in different ecosystems ever since the introduction. In understanding this species of snails‚ samples were collected‚ DNA’s were extracted‚
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under controlled lab conditions to observe how the crossover frequencies of S. fimicola may change under the “environmental stresses” of standard lab conditions. The general hypothesis for this experiment is that if increased crossover and mutation of DNA occurs in organisms when they are subjected to more extreme and variable conditions‚ then the S. fimicola strains grown in “optimal” and constant laboratory conditions should exhibit less crossover of genes. Without any pressing environmental stresses
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Edwin Southern invented the molecular technique of Southern Blotting in 1975. This technique is very useful because of its ability to detect a specific DNA sequence from a large amount of DNA ⎯ even from the whole genome. This specific sequence can be found through a combination of two different techniques: Agarose Gel Electrophoresis and Hybridization‚ which is known as Southern Blotting. This technique if performed in three phases: (I) prepare the gel and (II) make the blot‚ (III) hybridize and
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abundant and versatile macromolecule‚ is made up of a chain of amino acids connected by peptide bonds. Carbohydrates are sugars made up of monosaccharides connected by glycosidic bonds. Nucleic acids are made up of nucleotides‚ and examples include DNA and RNA. Each tissue in the body has a different amounts of the four macromolecules. Based on a study done by Baker‚ the percentage of lipid in adipose tissue in humans at birth is about 40% and increases up to about 75% with age (1). According to
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