Nervous System and Sensory Organs • dorsal roots and ventral roots - connect Spinal nerves to the spinal cord • medulla -responsible for many involuntary functions such as heartbeat and breathing‚ primary communication pathway between the spinal cord and the rest of the brain‚ • cerebellum - receives input from multiple sensory receptor types and uses this information in coordination of complex body movements • pons- communication between lower and higher brain regions • midbrain- processes
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p IB DIPLOMA PROGRAMME PROGRAMME DU DIPLÔME DU BI PROGRAMA DEL DIPLOMA DEL BI Biology Higher level and standard level Specimen paper 1s‚ 2s and 3s For first examinations in 2009 http://www.xtremepapers.net CONTENTS Biology higher level paper 1 specimen paper Biology higher level paper 1 specimen markscheme Biology higher level paper 2 specimen paper Biology higher level paper 2 specimen markscheme Biology higher level paper 3 specimen paper Biology higher level paper 3 specimen markscheme
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reduced efficacy of plant based medicines / drug formulations. To minimize this problem‚ genotype specific DNA markers need to be developed. Remaining unchanged through short term variations in environment at different locations‚ and also through different phase of life cycle‚ DNA fingerprinting patterns constitute dependable DNA markers for ultimate individualization of a biological entity. DNA fingerprinting patterns in addition to supplementing drug assessment protocol as also establishing authentic
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is the process of joining two DNA fragments or other molecules by a phosphate ester linkage with the action of enzyme. Ligation of DNA is process that involve the DNA of interest is inserted into the plasmid. This 3’-hydroxyl of DNA terminus are joined together with 5’-phosphoryl of another by phosphodiester bond. The ligation of DNA fragments usually performed by using T4 DNA ligase. All the reaction components such as ligation buffer‚ DNA insert‚ pGEM®-T‚ and T4 DNA ligase is mixed by gentle pipetting
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consists of three steps: • Denaturation: Samples of DNA with the target sequence are heated to a reaction temperature of 94-96˚ C that causes DNA melting of the DNA template without denaturing the enzyme by disrupting the hydrogen bonds between complementary bases of the double helical DNA‚ yielding single-stranded DNA molecules(“How Is PCR (polymerase Chain Reaction) Done?”). DNA polymerase does not get degraded in such high temperatures since the DNA polymerase used in this reaction is thermostable
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extraction and amplification of DNA (Mullis et. al. 1986). 2. PCR has the ability to isolate specific DNA sequences with the use of primers. This is done by denaturing the DNA (at 95o C) so it is able to anneal to the primers that specify a fragment to be amplified (Mullis et. at. 1986). These primes anneal to a specific sequence of DNA in order to amplify this desired sequence. Once the annealing process is complete‚ the primers are extended (at 30o C) by a DNA polymerase added to the reaction
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Cot Analysis of DNA Renaturation (single transition) In a DNA renaturation experiment the concentration of single strands remaining as a function of time is found to be 2nd order in single-strand concentration C. Integration between t = 0 and t yields: or which can be expressed as: Here C/Co is the fraction
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Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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Sample Methodologies for DNA Extraction and PCR for gender identification of Gallus gallus domesticus INTRODUCTION: DNA extraction and PCR have been used in numerous research projects‚ current research commonly utilise such techniques for the basis of their study and as economic cost of the proceduredecreases it will become even more prevalent in industrial and commercial settings. A variety of methods collection can be employed in order to extract DNA; the methods chosen can however directly
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Population Genetics of the Alu Insertion on the PV92 region of Chromosome 16 Abstract: PCR is a laboratory method used to amplify a small‚ specifically targeted‚ amount of DNA. It has three steps‚ the denaturing of the template DNA‚ the annealing of the primers to the DNA templates and the extension of the new DNA by Taq DNA polymerase. The Alu insert on the PV92 region of chromosome 16 is targeted and its frequency is measured according to the Hardy-Weinberg equilibrium in the Vanier HTK population
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