Preparation of SKEO The extraction of S. khuzistanica was performed by hydro distillation and clevenger apparatus for 5 hours. Once extraction was completed‚ the resulted essential oil was dehydrated using sodium sulfate (Merck). The essential oil was stored at temperature of 4ºC until using (12). In order to provide different concentrations of the extract‚ Dimethyl sulfoxide (DMSO) was used. Gas chromatography/mass spectrometry analysis of essential oil Isolation and measurement of the sample
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1) Using tweezers remove individual plants from the soil and remove the roots with scissors. Measure the weight of each individual and place in a 1.5 mL microfuge tube. Place this tube directly into liquid N2 to freeze plant tissue. 2) Remove tubes from the liquid N2 and quickly grind the plant tissue with a pestle. Add 300 L of Methanol 1%HCl and continue to grind the plant tissue. Allow extraction to occur overnight in a dark refrigerator. 3) Add 200 L Milli-Q H20 to each tube. Next
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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Abstract This work describes the extraction of caffeine from tea leaves to determine its % caffeine. The extraction process selectively dissolves one or more compounds in a mixture into an appropriate solvent. In this experiment‚ it was visible in the process wherein the components of the tea leaves were dissolved in two solvents‚ water and dichloromethane (DCM)‚ with DCM used for multiple extractions. The organic layer was evaporated and the determined % caffeine was 0.12%. Furthermore‚ the purified
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Extraction of Caffeine from Tea Leaves Introduction Caffeine is soluble in boiling water and as a result it is easily extracted from tea bags by steeping in hot water. This process leaves behind the water insoluble portions of the tea bag. However‚ water extracts more than just caffeine‚ so a final separation is done with an organic solvent that will dissolve primarily caffeine. The organic solvent used in this experiment is Dichloromethane (CH₂Cl₂). Dichloromethane is less polar than water
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Module 2 Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form
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Objective: To extract caffeine from tea powder using polar - nonpolar solvent extraction technique. Theory: The technique used to separate an organic compound from a mixture of compounds is called Extraction. Extraction process selectively dissolves one or more of the mixture compounds into a suitable solvent. The solution of these dissolved compounds is referred to as the Extract. Here the organic solvent dichloromethane is used to extract caffeine from an aqueous extract of tea leaves
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field; 25 but while men slept‚ his enemy came and sowed tares among the wheat and went his way. 27 So the servants of the owner came and said to him‚ ‘Sir‚ did you not sow good seed in your field? How then does it have tares?’ 28 He said to them‚ ‘An enemy has done this.’ The servants said to him‚ ‘Do you want us then to go and gather them up?’ 29 But he said‚ ‘No‚ lest while you gather up the tares you also uproot the wheat with them. 30 Let both grow together until the harvest‚ and at the
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Bio 1 Lab: Electrophoresis and DNA fingerprinting Jani Lynette Hagen October 31‚2014 U74644799 Electrophoresis is a technique which uses an electric field to separate molecules‚ allowing for identification and characterization of the molecules. It is commonly used to separate nucleic acids and protein molecules of various sizes. To prepare the gel for electrophoresis the amount of agrose needed must be calculated. For a 0.8 percent gel 0.8 grams of agrose is necessary per 100 ml of buffer. The DNA
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