reactions occur spontaneously‚ but generally some energy must be supplied to initiate the reaction; in other word an energy barrier exists between the reactants and the products. The “energy barrier” represents the activation energy of a chemical reaction. In this practical the activation energy of hydrolysis of p-nitrophenyl phosphate will be determined in the presence of the enzyme alkaline phosphatase. REAGENTS * A suitably diluted solution of alkaline phosphatase * 10 nM p-nitrophenyl
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Resting potential is created by a transport protein called the sodium-potassium pump. Resting potential occurs when ions are distributed unequally on the inside and outside of cells‚ and when cell membranes are selectively permeable to different ions. K+ is particularly important for the resting potential. The membrane is highly permeable to K+. In addition‚ the inside of the cell has a high concentration of K+ and the outside of the cell has a low concentration of K+. K+ will naturally diffuse
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Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate‚ as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore
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itself‚ it has energy. When you think of energy‚ you might think of action- or objects in motion‚ like the baseball that shatters a window. There are different forms of energy. Turn on an electric light and a dark room becomes bright. Turn on a music player and sound comes through your headphones. In both situations‚ energy moves from one place to another; effect and reaction. The two different types of energy are kinetic and potential energy. Kinetic energy is the energy a moving object has because
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Existential Therapy and Its Potential Applications Lorraine G. Daddario La Salle University Author Note [Include any grant/funding information and a complete correspondence address.] Abstract As a philosophically based model‚ existential therapy offers a fresh humanistic approach to psychotherapy‚ focusing on the overarching themes of human existence: inevitable death‚ freedom and its corresponding responsibility‚ existential isolation‚ and finding meaning in life. Through a genuine and authentic
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1 MARKET POTENTIAL ANALYSIS: A METHODOLOGY FOR ESTIMATING THE MARKET POTENTIAL FOR COMPUTERS AND OTHER INFORMATION TECHNOLOGIES Kenneth L. Kraemer and Jason Dedrick Center for Research on Information Technology and Organizations University of California‚ Irvine Irvine‚ CA 92697 May 1998 Working Paper: #ITR-149 This research was supported by grants from the U.S. National Science Foundation and IBM Global Services. 2 ABSTRACT This paper describes a methodology for estimating
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Nonconformity‚ Corrective Action and Preventive Action in EMS ISO 14001 The intent of 4.5.3 is that the organization put in place procedures for‚ 1) identifying actual and potential nonconformities to EMS requirements‚ 2) taking appropriate corrective or preventive action‚ and 3) reviewing the effectiveness of corrective or preventive actions taken. The nonconformity requirement of ISO 14001:1996 was a passive requirement in that it was only triggered when a nonconformity came to the attention of
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Determination of the Activation Energy of an Enzyme Catalysed Reaction PRACTICAL Report & Criterion Reference Grid * The expected sequence and details required for your practical report are outlined below. Please follow them closely‚ as they will facilitate the production of a structured report. * An introduction giving a synopsis of the experiment and the importance of rates of reaction and activation energy. This section should also state the aim of the practical (0.50-page) – 5 marks
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Determining activation energy (Ea) of a reaction © KCl http://hk.geocities.com/fatherofchemistry Objective To determine the activation energy for the reduction of peroxodisulphate(VI) ions. S2O82-‚ by iodide ions I-‚ using a ’clock’ reaction. Principle The equation for reduction of S2O82- by I- is: S2O82- + 2I- → 2SO42- + I2 The formation of iodine is ’monitored’ by small & known amount of thiosulphate ions‚ S2O32-:
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The importance of job descriptions is that it helps potential candidates to know what is expected of them and how they will be evaluated within the interview process. With job descriptions it can be of great value to employers as it helps them to relate potential candidates back to the job role and see if they fit well with the description of the ideal candidate they are looking for. Job descriptions are very useful for both Tesco and the potential candidates who are applying for the vacancy as it
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