1 The Limits of Amylase 2-1-14 Abstract This report explains the purpose of this experiment in a way that conveys information to the reader about Amylase’s ability to withstand acidic or basic pH. To do this‚ two test tubes were both filled with 5mL of a 5% amylase solution. The first one was filled with an acid‚ while the other was filled with a base. After dropping liquid Iodine and Benedict’s solution into each one‚ the tube with a basic pH tested positive for glucose. The acidic solution
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The role of salivary amylase in the digestion of starches remains controversial. In the absence of pancreatic amylase‚ the key enzyme for starch digestion‚ salivary amylase may well represent a potential compensatory alternate pathway for the digestion of amylose‚ amylopectin‚ and glycogen. Clinically significant depression of pancreatic amylase occurs in chronic pancreatitis‚ pancreatic resection‚ pancreatic neoplasm‚ cystic fibrosis‚ and other causes of pancreatic insufficiency. Quantitation
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“Is Starch Permeable in Cells? Abstract This report presents the weights of 3 samples of water with various amounts of solute after the use of osmosis. Three different tests were performed‚ each with a beaker of water containing varying amounts of starch from 30% concentration (12.5 grams of water) to 15% concentration (9.5 grams of water)‚ and then 0% (10.5 grams of water). To start this experiment‚ we put water into a make-shift dialysis tube‚ a type of semi-permeable membrane tubing made
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CHEM 1021 BREAKING DOWN STARCH USING SALIVARY AMYLASE Caution: You will be using a Bunsen burner and glassware to create your own constant water bath. Appropriate caution should be exercised when dealing with the Bunsen burner‚ hot water‚ and glassware. Purpose: Many plants store their energy in the form of starch‚ a polysaccharide made from repeating units of the monosaccharide glucose. Our bodies break down starch into the individual glucose units
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The effect temperature has on the activity of the enzyme α- amylase. Introduction Enzymes are a biological catalysts‚ which means that they speed up the chemical reactions in living organisms. Almost all of enzymes are energized protein molecules that catalyse and regulate nearly all biochemical reactions that occur within the human body. The reason in which enzymes are sensitive to heat‚ pH and heavy metal ions is because they are made up of proteins. The food we eat is turned into energy by enzymes
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Bio Lab 105 04 November 2013 The Effects Environmental Temperature and pH have on the Activity of Porcine Pancreatic Amylase Introduction There are many types of enzymes and each has a specific job. Enzymes are particular types of proteins that help to speed up some reactions‚ such as reactants going to products. One of them is the amylase enzyme. Amylases are found in saliva‚ and pancreatic secretions of the small intestine. The function of amylase is to break down big molecules of starch into
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Effect of varying Temperatures on Enzymatic Activity of Bacterial and Fungal Amylase and hydrolysis of Starch Abstract This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added
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Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme.
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The Effect of Starch on the Activity of Amylase with pH Variable Lab Report‚ Fall 2011 East Tennessee State University Department of Biological Sciences By: Shelby Brackett Date Performed: October 10‚ 2011 Lab Instructor: Joseph Kusi Biology 1111‚ Section 018 Abstract Enzymes are very important in chemical reactions. They are used to speed up the reaction taking place. They act by binding to a specific substrate and form an enzyme-substrate complex that may put stress on chemical bonds
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of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction temperature of
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