goal of this lab was conducted to determine the optimal temperature for bacterial and fungal Amylases and evaluate how temperature affects the catabolic rate of enzymes. Enzyme reaction rate was measured using an Iodine test in which drops of starch solution with either fungal or bacterial Amylase exposed to different temperatures were mixed with Iodine. Iodine is a dark blue color in the presence of starch and turns light yellow in its absence. Bacterial Amylase had an optimal temperature of 55°C‚ meaning
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sizes of molecules and how the chemical reactions take place. Therefore‚ the experiment was conducted using glucose and starch solution inside the dialysis tube. The starch and glucose that was put inside the dialysis tube help identify which of the two will reacted with potassium iodide inside the breaker‚ as the latter passed from the beaker into the tube‚ the glucose/starch solution’s change of color showed that the potassium iodide was small enough that it able to pass through from the solution
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relationship between temperature and the enzyme activity of amylase. This was achieved by attaining amylase enzyme‚ starch solution and potassium iodide (determines if enzymes hydrolyses the starch solution)‚ water bath and a hot plate. The temperatures used for this experiment were room temperature‚ 37oC‚ 60oC‚ 80oC‚ and 90oC. The hypothesis developed was that as the temperature increased‚ so will enzyme activity. Therefore‚ the ability of the enzyme to break down the starch solution will occur
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Purpose The purpose of the lab is to determine how different factors affect the digestion of starch. Starch is a type of complex carbohydrate with large polysaccharide molecules that are made up of hundreds of glucose subunits. The digestion of starch begins in the mouth with the enzyme amylase and continues in the small intestine. Maltose‚ a disaccharide‚ breaks down the large polysaccharide molecules. Maltase‚ also found in the small intestine‚ splits each maltose molecule
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Cynthia Vang Derrick 24 Title: Digestion of Starch Problem: Hypothesis: If synthetic saliva breaks down the cracker the most Then more of the cracker should be digested Because synthetic saliva contains amylase‚ and amylase helps break down complex carbohydrates. Interpretation: Data Table: The results of the iodine and Benedict’s tests for the Carbohydrate lab Test Tube contents and number Color of iodine test Color of Benedict’s test How much is present? 0= none 1= present
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of Human saliva (Salivary amylase) against Temperature Proponent: Ian Angelo P. Dela Cruz BS-Biology 1-3 Prof. McJervis S. Villaruel Professor – BIOL2015(Lab) ABSTRACT This report entitled “Enzymatic activity of Human saliva (Salivary amylase) against temperature” aims to know and observe the enzyme activity of the human saliva. The research only included the use of starch-agar as the medium to observe enzyme activity during the experiment. Five starch-agar plates were prepared
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Effect of different temperatures on amylase activity. Literature review This study is an attempt to follow the activity of amylase because it has a major role in the life of living organisms and is found abundantly in them. Amylase is a catalytic enzyme which hydrolyzes starch into maltose and dextrin at a certain temperature (Biology.kenyon.edu‚ 2015). In plants such as fruits and vegetables carbohydrates are referred to starch which is polysaccharide and is converted into disaccharide and eventually
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The Rate of Reaction that Enzyme Concentration‚ pH‚ and Temperatures Have on the Amylase Enzymes Color Disappearance Abstract: Compare reaction rates of the concentrations‚ pH’s‚ and temperatures of the enzyme Amylase. At what concentrates do the substrate molecules collide with each other‚ making the reaction possible? At what pH levels do the 3D molecular structures change breaking the H-bond and/or denaturize? At what temperatures do the collisions of the substrate molecules happen?
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Manila Philippines Abstract Salivary amylase‚ found in humans‚ is enzyme that catalyzes the hydrolysis of starch into simpler compounds. Its enzymatic activity is affected by several factors‚ such as temperature and pH. The rates of enzymatic activity of salivary amylase in different temperatures and pH were measured and resulted to be very near 50 C and 7 respectively. However‚ due to some errors that were committed‚ the expected optimum temperature was not achieved. Introduction Of all biomolecules
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Conclusion I predicted that if the decolorization of DPIP is caused by photosynthesis and not cell respiration and spinach extract containing chloroplasts and mitochondria is incubated with DPIP‚ then the rate of DPIP decolorization should be higher if in bright light verses dark light because DPIP is reduced by photosynthesis and not by the mitochondria or any other cellular function. If DPIP was only decolorized by chloroplasts‚ then the percent transmittance of chloroplast suspensions would be
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