Kadijat‚ and Kharla Ms. Bulahan Writer: Djina Jan-18-15 Lab #6 - DNA Extraction lab Introduction: DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Cytosine with Guanine. S ince DNA is the blueprint for life‚ every living thing contains DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis
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Procedure Access the virtual lab and complete the experiments. Part One (Flame Test): 1. Create and complete a data table for Part One of the lab. It should include the name of the element (or unknown) examined and the color of the observed flame: Barium-green Calcium-red Sodium-yellow Rubidium-purple Potassium-blue Lithium-pink 2. Identify each unknown from Part One of the lab and briefly explain why you identified each unknown as you did.: Unknown 1-yellow. I think it is Sodium because
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Physical and Chemical Change Objective: Recognize and distinguish between chemical and physical changes. Materials: - lab balance - microspatula - lab burner - dropper pipet - 5 test tubes - mortar and pestle - test tube rack - magnet - test tube holder - insulating pad - watch glass - safety goggles - glass square - lab apron or coat - copper sulfate pentahydrate - iron fillings - sodium chloride - magnesium ribbon - hydrochloric acid - paper
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Regents Chemistry Quarter 1 Midterm Review 1. The percent by mass of oxygen in Na2SO4 (formula mass = 142) is closest to 1) 11% 3) 45% 2) 22% 4) 64% 2. Given the unbalanced equation: __Al(s) + __O2(g) → __Al2O3(s) 3. 4. 5. 6. 7. balance the equation using smallest whole number coefficients. The percent by mass of aluminum in Al2O3 is approximately 1) 18.9 3) 47.1 2) 35.4 4) 52.9 What is the approximate percent composition by mass of CaBr2 (formula mass = 200)
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Strawberry DNA Extraction Donovan Roberts Mrs. Caudill Honors Biology 3/7/11 Introduction The structure of DNA is made of nitrogenous bases‚ phosphate groups‚ and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus‚ bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells
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Discussion The purpose of the Density Lab was to determine the identity of four unknown solids and two unknown liquids by calculating their densities and comparing them to a density chart‚ taking into account error analysis and finally classifying the substances. In order to calculate this density‚ we first found the mass of the container that was to be holding the substances. We then found the volume of the substance‚ and lastly determined the mass of the container and substance. We subtracted
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MULTIPLE EXTRACTION OF CAFFEINE FROM DRIED TEA LEAVES USING DICHLOROMETHANE Delos Reyes‚ K.‚ Dizon‚ G.J.‚ Enriquez‚ J.R.‚ Estrada‚ G. and Garcia C. Group 4 2G Med Tech Organic Chemistry Laboratory ABSTRACT Caffeine was extracted from dried tea leaves by multiple extraction technique. 10g of tea leaves was boiled in a solution of 4.4 g anhydrous sodium carbonate and 100ml distilled water and was extracted three times using 20 ml of dichloromethane. Theresidue was collected by decanting
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Name Lab Section GTA Station # 5. Extraction Pre-lab questions Complete the following questions and submit before beginning the experiment. 1. Which layer will be the aqueous layer when using dichloromethane (methylene chloride) as the solvent (i.e.‚ top or bottom)? Which layer will be the aqueous layer when using ether as the solvent? 2. When everything has been separated in Part D‚ which compounds will be in test tubes 1‚ 2‚ and 3?
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Anza Thomas 10/24/2013 CHE 261 (KC) Dr . McAtee Lab : Extraction of Caffiene Purpose: the purpose of this experiment is to extract caffeine from tea using solvent extraction techinques. Reference: Williamson‚ K.; Minard‚ R.; Masters‚ K. Macroscale and Microscale Organic Experiments‚ 2007‚ p. 137 to 171. Procedure: In a 50 mL beaker place 15 mL of water‚ 2.0059 g of sodium carbonate‚ and a wooden boiling stick. Bring the water to a boil on the ceramic heating plate. Remove the beaker
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Biotechnology Lab Report Lab: Extracting DNA from Bananas and Strawberries Purpose: To properly and successfully extract DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed
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