spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves in a
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E5 & E6 Extraction of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach.
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Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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Laboratory #16: Analysis of Grease in Water by Solvent Extraction Experiment Date: 2/19/10 Due Date: 2/26/10 II. Objective The goal of this lab was to determine the amount of impurity‚ in the form of oil‚ in water using the method of solvent extraction‚ also known as liquid-liquid extraction‚ a method of separating two materials with different physical and/or chemical properties. Two methods of evaporation will be used to finally determine the concentration of impurity- one using an explosion-proof
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Experiment 2: Extraction – Evaluation Summary Labs reports must be typed and chemical structures must be drawn with ChemDraw. Report must not exceed three pages (including this page). Page limit does not include any attached spectra or references. ** Deductions for hand written report/structures and exceeding page limit ** Report Breakdown Data/Results: _________________ /10 Discussion: _________________ /10 Report Total: _________________ /20 Other Lab Marks Performance:
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Title of Experiment Extraction of Spinach Date that the Experiment was Performed This experiment was performed on Wednesday‚ September 17th‚ 2014 at 2:45 pm in the St Ignatius Science Center Laboratory 323. Partners Names Taylor Jackson and Matt D’Angelo. Taylor‚ Matt‚ and I shared the same data. Purpose/Goals/Objectives The purpose of this experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to
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LAB # 5 Relationship Between Drop Height and Diameter of Plasticine Sphere PROBLEM: A student suggest that there is a proportional relationship between height at which a plasticine sphere is dropped and the diameter of the flattened part after dropping. Design an experiment to determine if this suggestion is true or not. AIM: To investigate the relationship between the drop height and the flattened part of a plasticine sphere after being dropped. HYPOTHESIS: As the height of the sphere plasticine
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COMMENT 343 DETERMINATION OF CHLOROPHYLL AND PHEO-PIGMENTS : SPECTROPHOTOMETRIC EQUATIONS~ It has been shown that chlorophyll degradation products may at times constitute a significant fraction of the total green pigments present in seawater (Yentsch and Menzel 1963; Lorenzen 1965; Yentsch 1965). These degraded forms‚ or inactive chlorophyll‚ absorb light in the red part of the spectrum; if they are present in concentrations significant relative to chlorophyll a‚ a serious error may be introduced
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Abstract This experiment focused on extracting and separating pigments of Chloroplast. For the procedure green leaves were grinded in a mortar with some chemicals and the fluid was filtrated to use for further analysis. Stripes of this solution were put on a filter paper and later‚ after dried placed into a beacon of solvent. After this the chloroplast pigments were separated by the solvent into groups of more or less soluble pigments. Aim How many pigment types are present in a green leaf? It
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Isolation‚ purification and characterization of alkaloid caffeine from tea leaves Date Submitted: September 3‚ 2014 Abstract Caffeine is one of the main constituents of tea leaves. In this experiment‚ 0.0039 g of pure caffeine was isolated from tea leaves. This was done through solid-liquid extraction followed by multiple liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together
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