Investigating the Enzymatic Activity of Catecholase through Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration University of Alabama at Birmingham Burgess‚ B.N. Introduction: Background Enzymes are macromolecules that act as catalysts in living organisms by speeding up chemical reactions without being changed or destroyed by the reaction (Campbell and Reece‚ 2008). Enzymes are able to speed up the rate of a chemical reaction by decreasing the activation energy during the reaction
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mainly the mitochondrial oxidative metabolism and cellular responses to bacterial invasion‚ xenobiotic and cytokines. To counteract the formation of ROS‚ a number of antioxidant proteins have been described. These proteins are superoxide dismutase‚ catalase‚ glutathione peroxidase‚ glutathione S-transferase‚ and ferritin heavy chain etc. Oxidative stress has been implicated to both the onset and progression of type I and type II diabetes ( ). The consequence of ROS production is associated with the
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Botany Lab ------------------------------------------------- Introduction This study observed the effects of different body fluids and solutions relative to breaking down bacteria‚ specifically in the human body. The enzymes we studied‚ lysozomes‚ help the body lyse‚ or break down bacteria by targeting peptidoglycan in bacterial walls. The solutions and fluids studied were saliva‚ mucus‚ tears‚ a stock solution of lysozomes‚ and distilled water. The solutions were placed in agar containing Micrococcus
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others
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| 143635 | Noraziemah Mohamad Noor | 145012 | Tittle : Processing of Yoghurt Objective : This experiment was carried out to: 1. Observe the significant difference between two different yoghurt processing techniques (namely acid and enzyme methods) by determining the physical property and sensory attributes of prepared yoghurt. 2. Provide the practical experience/overview of yoghurt production. 3. Collect‚ analyse and evaluate the experimental data. 4. Collaborate with companion
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Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase‚ an enzyme found in many different tissues‚ catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide
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synthesis‚ and in fireflies‚ also known as lightening bugs‚ bioluminescence (Biology Book). Bioluminescence is the process in which living organisms convert chemical energy into light (Branchini‚ 2008). In the fireflies case the reaction involves the enzyme luciferase which lights up a lantern-like mechanism under the tip the wings and attached to the end of the body of the firefly. It is presumed that the firefly uses the lantern to attract a mate. The goal in this experiment was to test the effect
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Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
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Physics Lab Report 1. For the wavelength measurement of different colors in the Hydrogen spectrum done in the lab‚ tabulate your data recorded along with the wavelength calculations performed for all colors in the spectrum. (2 points) Line Color a_left (m) a_right (m) a_average (m) sinq nm Red 0.235 0.27 0.2525 0.182145 5.47E-09 Green-Blue 0.17 0.33 0.25 0.180505 5.42E-09 Indigo 0.16 0.35 0.255 0.18378 5.52E-09 Violet? 0 0 0 0 0 To find the wavelength for all of the colors in this lab we used two
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