10-fold dilution of this ionic iron solution and 5 drops of concentrated HNO3 was made. This solution served as the source of ionic Iron for the remainder of the lab and was labeled “stock ionic Iron solution.” Next‚ a 50-mL aqueous ionic Iron and FerroZine® complex solution was prepared by adding 5.00mL stock ionic Iron‚ 3-mL of acetic acid buffer‚ 2-mL of 5% hydroxylamine hydrochloride‚ allowing five minutes for hydroxylamine to reduce Fe3+ to Fe2+‚ adding 2.5-mL of 0.01 M FerroZine® solution‚ and
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Chamberlin Date: 29 April 2017 Experiment Title: Acid-Base Titration Abstract: Vinegar is a common household item containing acetic acid as well as some other chemicals. This experiment is designed to determine the molar concentration of acetic acid in a sample of vinegar by titrating it with a standard solution of NaOH. CH3COOH(aq) + NaOH(aq) -> CH3COONa(aq) + H2O(l) By adding the sodium hydroxide‚ which is a basic solution‚ to the acetic acid‚ which is an acidic solution‚ a neutralization
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Determination of Ka for a Weak Acid Introduction In the experiment preformed the objective is to titrate a weak acid with a strong base. In a titration of a weak acid with a strong base the titrant is the strong base and the analyte is a weak acid. The reaction that will occur is the direct transfer of protons from the weak acid to the hydroxide ion. The data gathered will be represented on the titration curve‚ a graph of the volume of titrant being the strong base plotted against the pH .The
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Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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Characterization of Saponifiable Lipids and Characterization & Isolation of Complex Lipids Jan Kevin Roxas‚ Nashiba Samad‚ Erickson Santos Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Lipids building blocks of different organic material that are water insoluble. This can be divided into simple and complex lipids that have several forms like waxes‚ triacylglycerols‚ phosphoacylgylcerols‚ sphingolipids‚ eicosanoids‚ isoprenoids and many other. To identify‚
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because of the amount of time given in summer lab we had to save time and cut some days out. We were able to do this by being given our own Salicylic acid (SA) prepared for us instead of having to used wintergreen to make it and recrystallize it. In addition‚ we skipped the qualitative test of our purity for SA and Aspirin (ASA). The first part of this experiment is the preparation of ASA from SA were‚ we prepared our aspirin from the collected salicylic acid. A screw-capped vial was weighed empty to
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Lipids‚ which are also known as fats play a very important part in our bodies‚ it provides energy to producing hormones. As humans we wouldn’t be able to digest and absorb food without lipids. Eating more fat than we need can lead to weight gain but with proper amounts of lipids they are a healthy part of our diet. The main role of lipids in our body is to provide energy for muscles and body processes. Half of the fuel our body needs when at rest or with everyday activity comes from lipids. Lipids
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Ribozyme activity Answer: c 2. Which of the following are NOT directly required for translation? a. DNA template b. RNA template c. tRNA d. tRNA synthetases e. Ribosomes Answer: a 3. Which of the following is NOT needed to ensure that the correct amino acid is placed during the translation of a codon of the mRNA? a. Small subunit of the ribosome b. Large subunit of the ribosome c. tRNA d. tRNA synthetase e. Amino acyl site Answer: b 4. Which of the following is NOT an example of RNA editing? a. A-to-I
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Title: ACID BASE TITRATION. Objectives: 1. To determine the concentration of acid using titration. 2. Skills of titration techniques. Apparatus: 1. 250 volumetric flask 2. 10mL measuring cylinder 3. 25mL pipette 4. 50mL burette 5. 250mL beaker 6. 150mL conical flask 7. Retord stand 8. White tile 9. Stopwatch 10. Pipette bulb Chemicals: 1. HCl solution 2. 0.1M NaOH solution 3. H2SO4 solution 4. Distilled water 5. phenolphthalein
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