Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this
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Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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BioLab3 Lab Report 5 Enzymes Student Name: Cooper Lyon I. Enzyme Structure and Function EXERCISE 1 – Preparation of an enzyme activity standard At five minute intervals over the next fifteen minute period‚ record the color intensity of the solution of each test tube. Time (min) Tube S1 Potato Extract + Catechol Tube S2 Potato Extract + Water Tube S3 Catechol + Water 0 Shade of Yellow Clear/Milky Clear/Milky 5 Shade of Yellow Clear/Milky Clear/Milky 10 Orange Clear/Milky Clear/Milky 15
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Bibliography: Campbell‚ Neil.‚ Jane B. Reece. 2005. Biology‚ 7th ed. Beth Wilbur ed. Benjamin Cummings Publishing‚ San Francisco‚ CA. pp 833-834. Helms‚ Doris.‚ Carl Helms.‚ Robert Kosinski.‚ John Cummings.‚ 1998. Biology in the Laboratory‚ 3rd ed. Judith Wilson ed. W.H. Freeman and Company Publishing‚ New York‚ New York. pp. 38-14 – 38-16 LaFave‚ N. Virtual Water Flea Experiment.
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between light intensity and photosynthesis that could normally be seen had the experiment taken place without errors. The graph should show an increase in arbitrary units the further away from the light source the beaker is because the greater the distance from the light source‚ the lower the light intensity and so less photosynthesis would take place in the algal balls. This would result in more CO2 being present because it wouldn’t be being used up during photosynthesis. The decrease in CO2 in the
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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Aim: The aim of this experiment is to investigate the process of Electrophoresis and successfully analyse DNA fragments. Hypothesis: That the experiment will show the visual representation of DNA proteins‚ and that the shorter the band the further is will travel. Background: Restriction enzymes are DNA-cutting enzymes found in bacteria‚ which cut DNA into smaller fragments. A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides‚ known as restriction sites
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Kathleen Kramas Cells and Heredity Lab Tuesday 2:00-4:50 22 OCTOBER 2012 Membrane Permeability Decreases as Molecular Size Increases Introduction: Red blood cells are vital to organisms functioning properly. They are microscopic cells that carry oxygen from the lungs to all the tissues throughout the body. Upon transporting oxygen‚ red blood cells also exports waste‚ such as carbon dioxide‚ to the lungs where it can be expelled. Red blood cells are made up of hemoglobin which is surrounded by a
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Introduction: Mitosis occurs in all eukaryotic cell tissues and produces genetically identical daughter cells with a complete set of chromosomes. In humans‚ mitosis produces somatic cells that are diploid‚ which means they contain two non-identical copies of each of the twenty-three chromosomes. One copy is derived from the person’s mother and the other from the person’s father. Meiosis‚ on the other hand‚ occurs only in testis and ovary tissues‚ producing sperm and ova (eggs). The gametes that
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