Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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Specific Objective: Carry out test for reducing sugar‚ non reducing sugar‚ starch ‚ protein and lipid. BIOCHEMICAL TESTS Reducing and non-Reducing Sugars Sugars can be classified as either reducing or non-reducing based on their ability to reduce copper(II) ions to copper (I) ions during the Benedict’s Test. Reducing sugar have contain free aldehyde or ketone group and have the ability to reduce copper(II) ions to copper (I) ions during the Benedict’s Test. All monosaccharides are reducing
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their food. With the help of saliva produced by the salivary glands‚ which produce enzymes‚ food is able to be broken down further. This is also the place where ingestion takes place” “What are enzymes” “Enzymes are very efficient catalysts for biochemical reactions. In the mouth there’s an enzyme that will help your food undergo chemical digestion” “What is it” “It is amylase. Which help digest starch into smaller molecules. This enzyme will help break down the bread since it is filled of carbohydrates
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Isolation‚ Morphological‚ and Biochemical Characterization. A high-throughput screening of cartenoid-producing microorganisms resulted in 25 astaxanthin-producing bacterial strains from marine water samples that were collected from the Pacific coast of Japan (unpublished results). Among the isolates‚ a novel‚ highly selective astaxanthin-producing marine bacterium‚ labeled strain (N-5)‚ was isolated based on its ability to produce orange pigment on NA plate after 2 days of cultivation at 30 °C. Strain
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FLAME TEST Summary The purpose of this lab was to see how different compounds can change color while in a flame and with this color‚ I can identify the compound. The test involve putting sample element and see what color appears. Also Thermal excited electron goes from low to high energy level which raises the color but when the excitation ends electron gets to lower level which makes the color back to normal. In order to determine the identity of several unknowns‚ I will perform flame tests and
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Boake‚ Hannah Harper Various Color of Light as the Effect of Electron Emission Objective Observe the characteristic colors produced by certain metallic ions when vaporized in a flame. Identify the unknown metallic ions by means of its flame tests. Background When atoms absorb energy‚ electrons move into higher energy levels‚ and these electrons lose energy by emitting light when they return to lower energy levels. Energy levels are fixed energies that electrons can have. Ordinary light
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Flame Tests By: Jordan Anthony‚ Zack Balog‚ and Evan Degraw Regents Chemistry class‚ Union Springs High School‚ 4th period January 19/2015 The purpose of this lab was to see what colors are characteristic of particular metallic ions in a flame test and use their characteristic color to identify other elements. Another purpose is to understand how fireworks work. This experiment will help one understand how a flame test is performed and that every metal has a unique bright
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Aseel Muqbel 15/2/2015 11th D Flame Test Intro: The flame test is a procedure used by chemists to identify the presence of specified metal ions‚ based on the color the flame that appears along with it when it ’s heated. Our flame test was in lab class‚ where the main aim was to tested different solid metals in the flame in order to observe the wide variety of colors those chemicals also sometimes compounds (by mixing them) they produced‚ the second one was to identify unknown metals based
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Citrate Test: Some bacteria can use citrate as a source of carbon. To test if the unknown bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface
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Flame Test Lab Morgan Chemistry CP 5/30/14 Abstract- In the flame test lab‚ the flame test was performed to excite the electrons in the samples and observe the color of the flame. The flame emits a color because each element has an exactly defined emission spectrum‚ which one can use to identify them. For example‚ NaCl was highlighter yellow‚ Sr(NO3)2 was sun orange‚ CuCl2 was turquoise‚ LiCl was neon red‚ KCl was solar flare yellow‚ and BaCl2 was Voldemort green. Introduction- In Bohr’s
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