drops-no change in color The positive tubes were the tubes with the egg white‚ pepsin‚ and sugar. Protein was found in the first two substances. Biuret reagent can be used in a real life situation to test the detection of protein in food. The predicted results were the same as the results. Exercise 2: Testing for Sugar To add Benedict’s reagent to four substances‚ glucose‚ starch‚ onion juice‚ and distilled water‚ to test the detection of sugar in each. Test Sample Predicted Results Benedict’s
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NPTEL – Chemistry – Reagents and Organic reactions Module II Lecture 14 Reduction Reactions 2.1.1 Lithium Aluminum Hydride (LAH) 2.1.1.1 Introduction Lithium aluminum hydride (LAH) is a strong reducing agent with chemical formula LiAlH4. It can reduce a variety of functional groups such as aldehydes‚ esters‚ acids‚ ketones‚ nitriles‚ epoxides and azides. It vigorously reacts with water and all the reactions are performed in polar aprotic solvents. 2.1.1.2 Preparation It was first
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4-Dihydroxyphenylalanine CONDITIONS: METHOD: REAGENTS: A. 50 mM Potassium Phosphate Buffer‚ pH 6.5 at 25° C (Prepare 50 ml in deionized water using Potassium Phosphate‚ Monobasic‚ Anhydrous‚ Sigma Prod. No. P5379. Adjust to pH 6.5 at 25° C with 1 M KOH.) 1 mM L-Tyrosine Solution (Prepare 100 ml in deionized water using L-Tyrosine‚ Free Base‚ Sigma Prod. No. T-3754.) Tyrosinase Enzyme Solution (Immediately before use‚ prepare a solution containing 500 - 1‚000 units/ml of Tyrosinase in cold Reagent A.) T = 25° C‚ pH = 6
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= 17.70 µl) and HOBt (5 eq. = 36.48 mg) in the presence of TBTU (5 eq. = 86.70 mg) as a coupling reagent‚ DIPEA (5 eq. = 47 µl) as the base and DCM (10 ml) as solvent at room temperature. The mixture was stirred overnight. 3.3.3 Final Deprotection of protected peptide fragments According to different yielding efficiency‚ there are several
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Experiment 3: Wittig Reaction Introduction This experiment performs a modified Wittig reaction using a phosphorus-containing Hornes-Emmons-Wittig reagent to generate an enolate anion of trimethyl phosphonoacetate instead of a phosphorus ylide. The methyl trans-4-methoxy cinnamate produced is then analyzed using melting point and 1H NMR spectroscopy. Theory The Wittig reaction prepares alkenes from carbonyl compounds by attacking a phosphorus ylide with a nucleophilic carbon atom stabilized by
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Mucho Ado about Nothing In this essay‚ I will be exploring ways in which Kenneth Branagh adapts act 2 scene 1 in Mucho Ado about Nothing. In Act 2 Scene 1‚ instead of making it seem like a normal party which are usually held indoors‚ Branagh made it take place outdoors‚ at night time‚ which is lit up with a few candles/lights scattered around the party‚ it seem like it was made to be romantic‚ but also it is not too bright for them to be able to see as clear as day‚ and not too dark for them
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tube rack dropper bottles of 1% starch‚ 1% protein‚ 1% glucose‚ and distilled water (DW)‚ slurries of honey‚ egg white‚ and saltine crackers dropper of IKI solution (starch test) dropper bottle of Benedict’s reagent (glucose test) dropper bottles of 10% NaOH and 2% CuSO4 (Biuret Reagents – protein test) 95ºC water bath masking tape for labeling test tubes Procedure: Biuret test for a typical protein: Place a small piece of masking tape on 7 clean test tubes. Using a permanent marking pen
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based on the reduction of chromium(IV)‚ which is orange‚ to chromium(III)‚ which is green‚ when an alcohol is oxidized by the reagent. A change in color of the reagent from orange to green represents a positive test. Primary alcohols are oxidized by the reagent to carboxylic aicds; secondary alcohols are oxidized to ketones. Tertiary alcohols are not oxidized at all by the reagent. Hence‚ this reaction can be used to distinguish tertiary alcohols form primary and secondary alcohols. Procedures:
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Effects of Electrical Stimuli and Injected Reagents on Frog Hearts Melissa Higdon Section 05‚ Group 01 November 19‚ 2013 Introduction: The heart is a very complex muscle for all species. It is responsible for sending oxygenated blood throughout the body as well as sending deoxygenated blood to the lungs‚ and continuously circulate this way for as long as we are alive. Many things can be effected‚ for example how fast the heart beats or how much
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information on them (Benedict). The tapes did show in fact that he was covering up the crime‚ and he was greatly involved‚ but it however did not give any direct evidence to say that Nixon ordered the break in at the Watergate office (Genovese). It was guaranteed that Nixon was going to be impeached because of his involvement with the scandal and his attempt of covering up the crime. Nixon was far from interested in becoming impeached‚ so on August 9th‚ 1974‚ he resigned (Benedict). Many of Nixon’s
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