How Do I Make Cannabis Oil

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Lab Report 2

Introduction:

The purpose of this laboratory exercise is to acquaint the investigator with common methods used to identify carbohydrates and proteins in the laboratory.

Materials:

7 clean test tubes plus test tube rack
dropper bottles of 1% starch, 1% protein, 1% glucose, and distilled water (DW), slurries of honey, egg white, and saltine crackers dropper of IKI solution (starch test)
dropper bottle of Benedict’s reagent (glucose test)
dropper bottles of 10% NaOH and 2% CuSO4 (Biuret Reagents – protein test) 95ºC water bath
masking tape for labeling test tubes

Procedure:

Biuret test for a typical protein: Place a small piece of masking tape on 7 clean test tubes. Using a permanent marking pen, label the tubes 1-7. Add drops of solutions to each tube as follows:

|Tube # |Add 10 drops of: |Then add 10 drops of: |Then add 3 drops of: | |  |  |  |  | |1 |1% protein |10% NaOH |2% CuSO4 | |2 |DW |10% NaOH |2% CuSO4 | |3 |1% starch |10% NaOH |2% CuSO4 | |4 |1% glucose |10% NaOH |2% CuSO4 | |5 |honey |10% NaOH |2% CuSO4 | |6 |egg white |10% NaOH |2% CuSO4 | |7 |saltine crackers |10% NaOH |2% CuSO4 |

Colorimetric Tests

Note the color developed in each tube and then record your results in the laboratory report. The color developed in tube #1 represents a positive test for the presence of protein while the colors developed in tubes 2 through 4 represent negative tests for the presence of protein. The colors developed in tubes 5 through 7 vary depending on whether they contain protein.

[pic]Violet indicates the presence of protein.
|Substance tested |Color Produced |Check if Protein | | | |Present | |  |  |  | |1% protein |violet |√ | |DW |  |  | |1% starch |  |  | |1% glucose |  |  | |honey |  |  | |egg white |  |  | |saltine crackers |violet |√ |

Iodine (IKI) Test for a Complex Carbohydrate (Starch): After cleaning your test tubes, add drops of solutions to each tube as follows:

|Tube # |Add 10 drops of: |Then add 2 drops of: | |  |  |  | |1 |1% starch |IKI | |2 |DW |IKI | |3 |1% protein |IKI | |4 |1% glucose |IKI | |5 |honey |IKI | |6 |egg white |IKI | |7 |saltine crackers |IKI |

Note the color developed in each tube and then record your results in the laboratory report. The color developed in tube #1 represents a positive test for the presence of starch while the colors developed in tubes 2 through 4 represent negative tests for the presence of starch. The colors developed in tubes 5 through 7 vary depending on whether they contain...
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