A streak culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed
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citrate slant was then inoculated by lightly moving the tube in a swivel pattern to help the loop not touch the walls of the container. The Citrate slant was then incubated for 48 hours in a hot room of 37 degrees Celsius. Observations of any change or growth were properly recoded
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Microbial Growth of Consumable Products By Bryce Wilmott AIM: To calculate the quantity of observable microbial colonies on the surface of the Agar solid‚ as to determine the presence of microbes in consumable products i.e. yoghurt and blue vein cheese. HYPOTHESIS: Microbial growth will be present in two of the three Agar plates (those containing the food product) due to the suspected presence of microbes‚ whilst the control Agar plate (containing no food products)
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Introduction: Bacteria are microscopic‚ single-celled organisms. Their genetic information is encoded in one large chromosome. It can also be found in plasmids which are small circular pieces of DNA that contain important genetic information for the growth of bacteria. In nature‚ this information is often a gene that encodes a protein that will make the bacteria resistant to an antibiotic. The reason for this protein being made within the bacteria is because of how bacteria usually grow in the same
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showing that Unknown #7 could ferment glucose to acidic ends‚ Vogves Proskaer which tested negative because Unknown #7 failed to convert those acidic bi-products back to neutral ends‚ and Citrate which tested positive with a blue color change and growth on the medium because Unknown #7 was able utilize citrate. The Nitrate Reduction test displayed Incomplete Reduction with a red color change when Solution A and B were added meaning that it could only reduce NO3- to NO2-
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unknown bacterium and the methods are explained in detail in the lab handouts. THIS SHOULD BE IN PARAGRAPH FORMAT (I.E. LOOK AT JOURNAL ARTICLES FOR AN EXAMPLE) ALL ASSAYS MUST BE INCLUDED TO RECEIVE FULL CREDIT (Growth curve‚ CFUs (counting colonies/dilutions‚ can include equation)‚ Growth after incubating at RT for 6 days‚ MIC (antimicrobial disc)‚ Catalase‚ Oxidase‚ DNA isolation). Under each headline describe the methods and material. The example below includes for headline that should be included
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membrane‚ a nucleoid‚ cytoplasm‚ ribosomes‚ pili‚ and flagella. It is rod shaped and measures about 0.5 to 0.8 micrometer across by 2 to 9 mm in length. It occurs in chains and is non spore forming (Sanders 322). Lactobacillus acidophilus has optimal growth at 37-42 degrees
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Abstract This experiment analyzes the effects of how temperature affects bacterial and fungus amylase and also discovers the optimum temperature for these enzymes. The amylase was mixed with starch at temperatures of 0℃‚ 37℃‚ 57℃‚ and 90℃. Iodine was added to each mixture and colour changes in each case. Bacteria amylase was found to be effective at 55 0C as the temperature dropped drastically from 4.58℃ to 2.33℃. This shows that the amylase catabolized a lot of starch hence little is left which
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Bacterial Morphology MLT1 Task 11- Lab 2 Johnny Archuleta Western Governors University Question A and C answers. A wet mount stain is when a drop of water is placed onto the microscope slide. The water on the slide helps to support the organism and sample. The water fills the space between the cover slip and the slide. This action allows the light from the microscope to pass through the slide and the sample for better visualization of the organisms. A direct stain occurs when a charged
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C. Catalase test. 9. B. Streptococcus 10. B. Optochin (P disk) 11. this test is used to differentiate different types of alpha-hemolytic streptococci. 12. A. It is viridans streptococci 13. there was no inhibition of bacterial growth around the P disk. This is indicative of viridans streptococci. this is a collection of streptococci normally found in the oral cavity. 14. B. Type 2 is lactose-positive‚ and type 1 is lactose-negative. 15. C. Citrate test 16.
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