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Unknown Bacteria Staining Experiment

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Unknown Bacteria Staining Experiment
Lab Practical One Report
Unknown Bacteria Staining

Gram
Acid Fast
Spore

The first practical for microbiology was staining of an unknown bacteria. Professor Goode provided an unlabeled bacteria in which each individual were to conduct gram, acid fast, and spore staining tests from memory. When the gram staining test was conducted a positive results was obtained. When views on oil bacteria appeared purple and rod-like. I noted this result and looked at the lab key to see what the results narrowed my bacteria choices to. My possible results were now: Bacillus cereus, Mycobacterium smegmatis, or Lactobacillus acidophilus.
Next I preformed the acid fast staining. When preparing this slide and viewing it on oil immersion, I was a little more
…show more content…
It is known as a “friendly” bacteria and is a very common probiotic that is found naturally in our bodies, mainly in the mouth, intestines, and the vagina. Structure wise Lactobacillus acidophilus is a single-celled prokaryotic microorganism that lacks a distinct nucleus. It also contains a cell wall, a cytoplasmic membrane, a nucleoid, cytoplasm, ribosomes, pili, and flagella. It is rod shaped and measures about 0.5 to 0.8 micrometer across by 2 to 9 mm in length. It occurs in chains and is non spore forming (Sanders 322). Lactobacillus acidophilus has optimal growth at 37-42 degrees …show more content…
Overuse or a side effects that are currently known about this bacteria is constipation and flatulence. With Lactobacillus acidophilus there wasn’t much about the production of toxins it could cause, but more about the detoxing abilities. It aids in producing enzymes which promote digestibility, decreases the levels of toxic amines in the blood, the positive list goes on. When researching of other bacteria that was closely related to Lactobacillus acidophilus. I was unsuccessful. But because it does grow naturally within us this bacteria works in conjunction with other bacteria and organisms within our

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