Beta-Galactosidase and Western Blot 0 1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes‚ namely‚ _lacZ_‚ _lacY_ and _lacA_‚ the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_‚ _lacY_ and _lacI_ would code for beta-galactosidase‚ galactosidase permease
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Chapter 1 MULTIPLE CHOICE QUESTIONS Topic: Atomic Orbitals 1. A) B) C) D) E) In quantum mechanics a node (nodal surface or plane) is: a place where Ψ is negative. a place where Ψ is positive. a place where Ψ = 0. a place where Ψ2 is large. a place where Ψ2 is negative. Ans: C Topic: Atomic Orbitals‚ Molecular Orbitals 2. When the 1s orbitals of two hydrogen atoms combine to form a hydrogen molecule‚ how many molecular orbitals are formed? A) 1 B) 2 C) 3 D) 4 E) 5 Ans:
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Exp.11) Identification of unknown ketones. Introduction: Given five samples of a known ketone derivative‚ the purpose of this experiment is to identify which unknown ketone derivative corresponds to the five known samples. In other words‚ using specific methods of compound detection‚ it is possible to match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment‚ identification of the unknown ketone is accomplished through thin
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Keghan Chapter 8 MULTIPLE CHOICE QUESTIONS Topic: Structure Elucidation 1. An alkene adds hydrogen in the presence of a catalyst to give 3‚4-dimethylhexane. Ozonolysis of the alkene followed by treatment with zinc and acetic acid gives a single organic product. The structure of the alkene is: CH3 A) CH3CH=C-CHCH2CH3 (cis or trans) CH3 CH3 B) CH3CH2C=CCH3 (cis or trans) CH2CH3 C) CH3 CH2=CCH2CHCH2CH3 CH3 CH2 D) CH3CH2CCHCH2CH3 CH3 CH3 E) CH3CH2CHCHCH=CH2
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TEA Dr. B.L. Wedzicha of t h e Procter D e p a r t m e n t of Food Science‚ U n i v e r s i t y of Leeds‚ describes t h e c h e m i s t r y of black t e a manufacture Tea is the m o s t w i d e l y c o n s u m e d beverage in the w o r l d . The economic importance of an annual w o r l d production of tea estimated to be in the region of 1-1·5 million tonnes has resulted in considerable attention being paid to the understanding of the chemical and physical changes w h i c h take place during tea
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Reece). Spinach leaves contain chlorophyll a and b and b -carotene as major pigments as well as smaller amounts of other pigments such as xanthophylls. The two forms of chlorophyll are identical except that a methyl group in a is replaced by an aldehyde in b. In terrestrial plants‚ the most important pigments are chlorophyll a (blue-green)‚ chlorophyll b (yellow-green)‚ xanthophyll (yellow) and carotene
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SPRAYKE: A MIST OF CAKE An Entrepreneurial Research Project Submitted to the Faculty of the University of Perpetual Help System – Jonelta City of Biñan‚ Laguna In partial fulfillment of the requirements for the degree of Bachelor of Science in Accountancy CHAPTER 1 THE PROBLEM AND ITS SETTING Introduction “Fragrance has a power of persuasion stronger than that of words‚ appearances‚ emotions‚ or will
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FMDC 2012 ENGLISH Q ambiti0n is one of those __which is never satisfied. A) ideas B) fancies C) energies D) passions Q. The opponents were outnumbered‚ but still the commander refused to give...? A) out B) way C) over D) in Q. spot the error a. The dean of this college is a good friend of me. b. There have been heavy rainfall yesterday! Q. choose similar meanings a. barbarian (uncivilized‚ civilized) b. rude Q. choose opposite meaning
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Alkynes Chapter 7 Triple Bond Structure Nomenclature IUPAC: use the infix -yn- to show the presence of a carbon-carbon triple bond 4 3 2 1 2 3 4 6 7 5 1 2 3 4 6 7 5 1 3-Methyl-1-butyne 6‚6-Dimethyl-3-heptyne 1‚6-Heptadiyne Common names: prefix the substituents on the triple bond to the name “acetylene” IUPAC name: Common name: 2-Butyne D imethylacetylene 1-Buten-3-yne Vinylacetylene Physical Properties
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Three-Dimensional Structure of Proteins Rotation around the -Carbon in a Polypeptide A Sterically Nonallowed Conformation The Helix and Pleated Sheet Conformationally allowable structures where backbone is optimally Hbonded (linear Hbonds). Helix (3.613 Helix): •3.6 residues/turn •Rise = 0.15 nm/ residue •13-atom hydrogenbonded loop Linus Pauling and Robert Corey‚ 1950 Pleated Sheet: •Anti-parallel or parallel •2.0 residues/”turn” •0.34 nm/residue (anti-parallel) or 0.32 nm/residue
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