Hypothesis: The hypothesis for this particular practical experiment is that‚ as the pH decreases or increases from neutral pH of approximately 7‚ the amount of microbial growth on the agar solution will decrease Materials: Agar Solution Preparation: *hand sanitizer/ hand wash soap *small container *1g of agar powder *0.25g of beef extract stock *60mL of heated water *electronic balance *small spoons (2x) *teaspoons (2x) *0.2M Acetic Acid Solution (pH of 3) *0.2M Sodium Hydroxide Solution
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Ex.No.3 | PROCESSING OF JAM AND JELLY | Aim: To prepare jam and jelly from the given fruits and evaluate the same. Introduction Jam is prepared by boiling the fruit pulp with sufficient quantity of sugar to a reasonably thick consistency‚ firm enough to hold the fruit tissues in position. FSSAI - Food Safety and Standards Authority of India (2006) previously FPO - Fruit products order (1955) 45 per cent of fruit pulp should be used for every 55 per cent of sugar and the prepared
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Contributors to Bacteriology In an unassuming way‚. they moved agar from the kitchen to the lab‚ revolutionizing bacteriology WOLFGANG HESSE T RANSLATED BY D IETER H. M. GR~SCHEL Walther Hesse was a well-known community health physician in the Kingdom of Saxony‚ a student of Max von Pettenkofer‚ the father of hygiene‚ and of Robert Koch‚ the father of medical microbiology. His American wife‚ Fanny Angelina‚ introduced agar-agar to the new science of microbiology. The Hesse Family Walther
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certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth‚ shaded mulch‚ and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The bacteria were then left to grow on their own in a relatively well shaded area. As a control‚ a cotton swab that had nothing on it was used. It was recognized that different types of bacteria exist in different environments and grow in different
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Materials • 30 petri dishes • Nutrient agar (a culture containing agar used to grow bacteria) • Sterile paper disks • Bacillus subtilis broth • Home-built incubator (a sealed box with a heat pad underneath) • Light box • Penicillin • Streptomycin • Tetracycline • Glutaraldehyde • 5 percent phenol alcohol • Fresh garlic extract Procedures 1. Divide petri dishes into six groups of five. 2. With adult supervision‚ melt the agar medium. 3. When liquid agar cools‚ pour an equal amount into each
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Question: How does the size of the cell affect its efficiency in exchanging substances through several ways‚ like diffusion? Aim: To plan and carry out an investigation to show the relationship between volume/Cm3‚ surface area‚ and diffusion using agar cubes measured in time/s; and to demonstrate‚ using diffusion‚ why the size of cells is limited‚ keeping the room temperature and pressure constant. Hypothesis: I expect to find that when the surface area to volume of a cell reaches a certain level
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- 3 Petri dishes prepared with agar - 1 disinfected swab - 1 bottle of disinfected water - A piece of filter paper - A hole puncher - 4 test tubes - 1 measuring cylinder - 1 pipette with disposable tips - Tetracycline - Clindamycin - Benzoyl peroxide - 1 beaker of water - P. acne bacteria culture - 1 forcep - 1 digital weighing scale - 1 marker pen 1. Before starting the experiment‚ make sure you clean your work area with Chlorox and wear gloves at all
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satisfactory method employed to evaluate the effectiveness of antiseptic agents against selected test organism. In this method‚ sterile filter paper disk is impregnated with an antiseotic and placed on fresh heavily inoculated agar plate of a test organism. Following incubation‚ the agar plate is looked for a zone of inhibition. The presence of a clear zone of inhibition surrounding the disk is indicative of inhibitory (antimicrobial) activity against the organism. This experiment deal with the evaluation
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differed between my group’s nutrient agar and MAC agar plates. In courgette‚ there was moderate growth in nutrient agar while it was heavy in Mac agar. In beef broth‚ there was slight growth in nutrient agar while it was negative in MAC agar. In fresh basil‚ growth was heavy in nutrient agar while it was heavy in MAC agar. Where cottage cheese was the ingredient‚ both plates had heavy growth. For walnut‚ growth was slight in nutrient agar while it was negative in MAC agar. Generally‚ the slight difference
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Diffusion in agar cubes Introduction: All cells are dependent on a processes called diffusion‚ which is the movement of a substance from an area of higher concentration to an area of lower concentration. Cells need to obtain certain essential substances‚ like oxygen‚ for their survival. The rate of diffusion is said to be dependent upon the various factors: Size of molecules that have to diffuse‚ concentration gradient‚ the distance across which diffusion occurs‚ number of pores/openings‚ surface
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