Preview

Proteus Vulgaris Essay Example

Good Essays
Open Document
Open Document
459 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Proteus Vulgaris Essay Example
Proteus Vulgaris

Proteus vulgaris is a gram negative, facultatively anaerobic, bacillus rodchemoheterotroph bacterium belongs to the family Enterobacteriaceae. It possesses peritrichous flagella, making it actively motile.
P.vulgaris occurs naturally the intestional tracts of humans and animals, soil, fecal matter, polluted water, and raw meat. It is grouped with the enterobacteriacea and is an opportunistic pathogen of humans. In humans P.vulgaris is known to cause urinary tract infections and wound infections.
P.vulgaris is associated with nosocomial infection, and has the ability to degrade urea to ammonia by production of the enzyme urease.

McConkey agar contains lactose, which P.vulgaris does not ferment. It ferments glucose, sucrose, galactose, glycerol occasionally maltose with gas production, but never lactose. P.vulgaris ferments liquefies gelatin, casein, and blood serum, curdling milk with acid production. P.vulgaris provides a positive result for: sulfur reduction,urease production, tryptophan deaminase production, and indole production. It’s not limited to any specific temperature range, but good growth occurs at 20 to 30 degrees Celsius, while growth is poor at 37 degrees Celsius. P.vulgaris has two interesting features. The cells are highly motile and swarm across the surface of the agar plates, forming a very thin film of bacteria. When the cells stop and undergo a cycle of growth and division, the swarming periods are interspersed wth periods and the colony has a distinct zonation.
The other feature is that. P.vulgaris can produce urease and degrade urea to ammonia. By alkalining the urine, P.vulgaris makes the environment more suitable for its survival. P.vulgaris is more prone to cause nosocomial infections. To prevent transmission of nosocomial pathogens within hospitals, the persistence of nosocomial pathogens on surfaces was assessed. The longer a nosocomial pathogens

You May Also Find These Documents Helpful

  • Good Essays

    Indole Test Lab Report

    • 1279 Words
    • 6 Pages

    pneumoniae does not produce proteases and cannot break down proteins via proteolysis. The fat hydrolysis test was performed to determine whether our bacteria produces lipase, an enzyme that breaks down fat. The fat hydrolysis test was positive, showing K. pneumoniae produces lipase and can break down fats. The indole test was performed to determine if our bacteria can break down tryptophan via the enzyme tryptophanase. Our indole test came back negative, meaning K. pneumoniae does not produce tryptophanase and does not break down tryptophan into indole, ammonia, and pyruvic acid. The urea test was performed to determine if urea is hydrolyzed via the enzyme urease. The urea test was positive, meaning K. pneumoniae produces urease to break down urea. An inoculation onto Kligler’s iron agar determines if an organism can ferment glucose and lactose, it also detects the production of hydrogen sulfide from the breakdown of cysteine. Our Kligler’s iron agar showed acid with gas production, meaning K. pneumoniae fermented both glucose and lactose. The hydrogen sulfide production was negative. A Litmus Milk test is done to determine whether the organism can ferment lactose, digest the milk proteins using proteases, cause the…

    • 1279 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Gram Staining Lab

    • 423 Words
    • 2 Pages

    Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria on it to see if the bacteria obtained is gram positive or gram negative. My hypothesis is there will be a lot of bacterial growth on all of the plate.…

    • 423 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Three tests were performed to determine the identity of unknown number 34, a gram stain, citrate utilization test, and a urease detection test. These tests are explained in detail, and the method of performing the tests is described. The tests showed that the bacterium was gram-negative bacilli that did not utilize citrate, and the ability to hydrolyze urea using urease. These results indicate that the identity of unknown number 34 is Proteus mirabilis.…

    • 1550 Words
    • 7 Pages
    Good Essays
  • Powerful Essays

    This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose, glucose, and lactose), the Urease Test, the Fermentation of Lactose Test, the Sulfide Indole Mobility (SIM) Test, the Nitrate Reduction Test, the Protein Hydrolysis Test, the Catalase Test, and the Cytochrome Oxidase Test. The microbes that were tested during this lab were: Escherichia coli, Bacillus cereus, the unknown, Proteus vulgaris, Staphylococcus epidermis, Enterobacter aerogenes, the control, and Pseudomonas fluorescens. The microbes tested during these various tests were looking for which would: reduce sulfur/produce sulfate, produce indole, or possess motility, reduce nitrate, and contain protease, catalase and oxidaase.…

    • 2351 Words
    • 10 Pages
    Powerful Essays
  • Good Essays

    Unknown Lab Report

    • 673 Words
    • 3 Pages

    Since P. aeruginosa is an opportunistic organism, most of the people that are affected by it are people whose immune systems are compromised, which is why P. aeruginosa is such a big problem in hospitals and clinics. The best way to control this pathogenic organism is by proper isolation procedures, aseptic technique, and careful cleaning of equipment. Using aseptic technique and cleaning all surgical equipment properly is very important because P. aeruginosa is easily spread in hospitals by nurses, surgeons and other hospital…

    • 673 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Procedure: Obtained a small Styrofoam cooler placed two small light bulbs in side and observed temperature over 24 hours to ensure temperature could be maintained between 98-100 degrees. Using a 10% bleach solution I then cleaned my work area. Transferring one capsule of L. acidopholis into a tube of MRS broth using the aseptic transfer technique then marked a line on test tube to record sediment. Labeled tube of nutrient broth S. epidermidis, then using a sterile swab obtained sample of bacteria from skin then transferred using the aseptic transfer technique into the sterile media. Incubated both specimens for 48 hours observed and recording results of growth at 24 and 48 hours. After observing final growth pattern at 48 hours prepared both wet mount and direct stain slide for each of the cultures. Viewed under microscope using both the 40X and 100X oil immersion lens. Disinfected work area.…

    • 768 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Pili that allows the structure to attach onto other cells like a leech. The capsule which protects the cells from the white blood cells that attack foreign invaders. Endospores which allow them to survive rough conditions.…

    • 316 Words
    • 1 Page
    Satisfactory Essays
  • Good Essays

    Citations: and References shall be in American Society of Microbiology (ASM) style. (-25 points if you do not properly use ASM style)…

    • 1226 Words
    • 5 Pages
    Good Essays
  • Good Essays

    The media was inoculated with a single stab to the center of the tube and incubated. After incubation, the growth was restricted to the stab line, the bacteria is nonmotile.…

    • 698 Words
    • 3 Pages
    Good Essays
  • Better Essays

    The importance of identification of a certain microorganisms can range between a life threatening diseases to a creation of certain antibiotic. Understanding the principals of living microbes and identifying my unknown bacteria through numerous biochemical and metabolism tests, with the outmost confidence, Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the experiments and comparing them to the other six known bacteria: Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Alcaligenes faecalis, Escherichia coli, and Proteus vulgaris that were used in the lab, as well as comparing and contrasting the actual and factual results.…

    • 2943 Words
    • 12 Pages
    Better Essays
  • Satisfactory Essays

    Ding Dong Worksheet

    • 773 Words
    • 3 Pages

    What is the infection agent (pathogen) that causes this infectious disease? For example the name of the bacteria, virus or parasite.…

    • 773 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    The Scarlet Ibis Ever wondered about the way someone special to you can be taken from the world in a heartbeat? How everything would change in that moment? The story, “The Scarlet Ibis,” demonstrates the theme that you should never take anyone for granted. In this story, the symbolism and the characterization of Brother and Doodle strongly supports the theme of the story. They are both great contributors to the development of the theme of The Scarlet Ibis. To begin with, the purpose of this theme is to inform the audience that you should never take anyone for granted. No matter what they do or who they are to you, someday you might not have them anymore and you will feel very guilty. Just like Brother in The scarlet ibis. He treated Doodle very rough in the beginning. But towards the end of the story, his love for him grew. Although at the end of the story, Brother only saved himself from the storm, and not his brother Doodle. When the storm hit, Brother ran faster than Doodle could, because doodle had a disorder. Doodle was left behind screaming for help when the storm took him. By the time brother went back to him it was already too late. Guilt ran over brother.…

    • 565 Words
    • 3 Pages
    Good Essays
  • Good Essays

    What is an example of a real life outbreak of this foodborne illness in the United States?…

    • 360 Words
    • 1 Page
    Good Essays
  • Powerful Essays

    The nosocomial infection prevention program has been developed to serve as basic practical resource to all health care individuals, as well as for those who work in the infection control department in our health care facility. The program will provide helpful information, rationales and recommendations in our join effort to fight hospitals’ acquired infection. This program will assist our hospital administrators, infection control personnel and, those involved in direct patient’ care to prevent hospital acquired infections.…

    • 1041 Words
    • 5 Pages
    Powerful Essays
  • Powerful Essays

    Stanton,S.L. and Dwyer, P.L. 2000 in Bardsley, A. Urinary tract infections: prevention and treatment of a common problem Nurse Prescriber 1(13) pp113-117…

    • 6153 Words
    • 25 Pages
    Powerful Essays