What Is Saraca Acoca, A Significant Antimicrobial Activity?
Saraca acoca, a potential candidate having a significant antimicrobial activity and widely used in the field of ayurvedic medicine. (Pradhan .P et al., 2009). especially, used in treatment of infertility (Roya R et al., 2006). The nanoparticles of silver ranges from 10-100nm and they have very high surface to volume ratio. They have very good solubility, stability and also excellent optical, electrical and thermal properties. Silver nanoparticle are off different size and shape depending on the method of fabrication. As the size reduces, the area exposed is higher and thus the exposed atoms communicates well with its surroundings, this forms the basis for its excellent antimicriobial activity. The physical and chemical method
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2013), (Logeshwari P et al., …show more content…
The studies of size, morphology and composition of the nanoparticles were performed by means of transmission electron microscopy (SEM) and Fourier transform infrared (FTIR) spectral measurements were carried out to identify the potential biomolecules in Saroca asoca leaf extract which is responsible for reducing and capping the bioreduced silver nanoparticles. Samples for SEM studies were prepared by placing drops of the silver nanoparticles solutions on carbon-coated SEM grids. (Okafor F et al., 2013)
Antimicrobial activity of the Green Synthesized Nanoparticle
The organisms used for testing were Escherichia coli, Staphylococcus sp, Streptococcus sp, Pseudomonas sp, Proteus sp, Bacillus sp, Aspergillus sp and Candida sp. Overnight cultures were prepared with nutrient broth.
Preparation of plates and antimicrobial testing
Muller- Hinton Agar (MHA) for antibacterial testing and SDA for antifungal testing were prepared ,autoclaved and the media was poured onto the petriplates and allowed to solidify, each organism was swabbed onto the plates under sterile conditions.The antimicrobial testing was done in well method where the samples were loaded in the well. The plates were incubated @ 37˚C for 24hr and the zone of inhibition was observed.The diameter of the zone was measured in
2013), (Logeshwari P et al., …show more content…
The studies of size, morphology and composition of the nanoparticles were performed by means of transmission electron microscopy (SEM) and Fourier transform infrared (FTIR) spectral measurements were carried out to identify the potential biomolecules in Saroca asoca leaf extract which is responsible for reducing and capping the bioreduced silver nanoparticles. Samples for SEM studies were prepared by placing drops of the silver nanoparticles solutions on carbon-coated SEM grids. (Okafor F et al., 2013)
Antimicrobial activity of the Green Synthesized Nanoparticle
The organisms used for testing were Escherichia coli, Staphylococcus sp, Streptococcus sp, Pseudomonas sp, Proteus sp, Bacillus sp, Aspergillus sp and Candida sp. Overnight cultures were prepared with nutrient broth.
Preparation of plates and antimicrobial testing
Muller- Hinton Agar (MHA) for antibacterial testing and SDA for antifungal testing were prepared ,autoclaved and the media was poured onto the petriplates and allowed to solidify, each organism was swabbed onto the plates under sterile conditions.The antimicrobial testing was done in well method where the samples were loaded in the well. The plates were incubated @ 37˚C for 24hr and the zone of inhibition was observed.The diameter of the zone was measured in