Use of Micropipette and Spectrophotometer
Group No. 10 Date Started: June 11, 2013
Tongco, Val Justin DM. Date Finished: June 18, 2013
Santos, Erickson S.
Vasquez, Jona Candace P.
Wong, Victor Lorenzo E.
Experiment 1
Use of Micropipettor and Spectrophotometer
Introduction
In scientific experiments, correct measurements are required to achieve precise and accurate data. Precision is the degree to which several measurements provide answers very close to each other. Accuracy describes the nearness of a measurement to the standard or true value. Certain measuring devices are used to attain precision and accuracy.
One example is the Air displacement pipette or the micropipettes. The micropipettes are measuring tools used to transfer liquid in the microliter scale. They are known to be the most precise and accurate of all pipettes. [1] In using the micropipette, when you push down the plunger of the micropipette, a first stop will indicate the resistance. The second stop generates the force applied to the liquid. The first stop is used to suck up the correct volume of the liquid. The second stop is used to completely expel the liquid you are measuring. [2] The micropipette uses the air displacement or the vacuum principle.
This experiment aims to introduce the general uses of micropipettes and the differences and importances of Precision and Accuracy.
Materials and Methods
Testing accuracy and precision in using a micropipettor Bromphenol blue [1.25% (w/v)], 10 microcentrifuge tubes, micropipettors and tips, GENESYS TM 10 uv-vis spectrophotometer, vortex mixer, semi-micro cuvettes (plastic or glass) and distilled water were the materials used in this experiment. In testing the accuracy of operators, first, the spectrophotometer was set at 540nm. Second, 1mL of distilled water was placed in each of five microcentrifuge tubes. Third, using micropipettors, the following amount of bromphenol blue was added to each successive tube: 0.5, 1.0, 1.5, 2.0, 2.5µL. Only one member was
Tongco, Val Justin DM. Date Finished: June 18, 2013
Santos, Erickson S.
Vasquez, Jona Candace P.
Wong, Victor Lorenzo E.
Experiment 1
Use of Micropipettor and Spectrophotometer
Introduction
In scientific experiments, correct measurements are required to achieve precise and accurate data. Precision is the degree to which several measurements provide answers very close to each other. Accuracy describes the nearness of a measurement to the standard or true value. Certain measuring devices are used to attain precision and accuracy.
One example is the Air displacement pipette or the micropipettes. The micropipettes are measuring tools used to transfer liquid in the microliter scale. They are known to be the most precise and accurate of all pipettes. [1] In using the micropipette, when you push down the plunger of the micropipette, a first stop will indicate the resistance. The second stop generates the force applied to the liquid. The first stop is used to suck up the correct volume of the liquid. The second stop is used to completely expel the liquid you are measuring. [2] The micropipette uses the air displacement or the vacuum principle.
This experiment aims to introduce the general uses of micropipettes and the differences and importances of Precision and Accuracy.
Materials and Methods
Testing accuracy and precision in using a micropipettor Bromphenol blue [1.25% (w/v)], 10 microcentrifuge tubes, micropipettors and tips, GENESYS TM 10 uv-vis spectrophotometer, vortex mixer, semi-micro cuvettes (plastic or glass) and distilled water were the materials used in this experiment. In testing the accuracy of operators, first, the spectrophotometer was set at 540nm. Second, 1mL of distilled water was placed in each of five microcentrifuge tubes. Third, using micropipettors, the following amount of bromphenol blue was added to each successive tube: 0.5, 1.0, 1.5, 2.0, 2.5µL. Only one member was
References: Retrieved from http://webphysics.iupui.edu/NH/Projects/TEAMS%5B2%5D/err6.htm at June 15, 2013, 9:00 PM Retrieved from http://wserver.scc.losrios.edu/~nussl/chem401/LABS/Lab%2001%20Pipetting.pdf at June 17, 2013, 8:30 PM