Toxicology of Propoxur

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Toxicology of Propoxur
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Pharmacology 1 – Group B3A
List of Contibutors:
Ali Mourad
10747691
16/4/2013
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Toxicology of Propoxur
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Pharmacology 1 – Group B3A
List of Contibutors:
Ali Mourad
10747691
16/4/2013

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Abstract
The purpose of this analysis is to investigate the effect of application of propoxur (a carbamate insecticide) on the American cockroach. From experimental observations, it has been concluded that the amount of propoxur needed to kill 50% of the test subjects (referred to as LD50) was 0.0487(%w/v) which deduces to be 0.22012mg per cockroach (calculated for 0.452grams of cockroach). The conclusions were drawn immobilising the American cockroach with ice then applying propoxur to be incubated at 23 degrees Celsius. Literature findings in the past were sourced and results were compared with the current study. Abstract

The purpose of this analysis is to investigate the effect of application of propoxur (a carbamate insecticide) on the American cockroach. From experimental observations, it has been concluded that the amount of propoxur needed to kill 50% of the test subjects (referred to as LD50) was 0.0487(%w/v) which deduces to be 0.22012mg per cockroach (calculated for 0.452grams of cockroach). The conclusions were drawn immobilising the American cockroach with ice then applying propoxur to be incubated at 23 degrees Celsius. Literature findings in the past were sourced and results were compared with the current study.

Toxicology of Propoxur
introduction
introduction

Figure 1: Chemical structure of Propoxur

Figure 1: Chemical structure of Propoxur

The American cockroach (Periplaneta Americana) or more commonly identified as the waterbug is the largest species of the common cockroach. It is considered to be a peri domestic pest and is common in tropical habitats. The P. Americana particularly common in Southern United States and has spread worldwide including Greece, India and Taiwan especially in the subtropics and tropics. The P. Americana is reddish, brown in appearance and an adult grow up to 4cm in length. Its habitat encompasses warm, humid and shady habitats and can live on average 4 to 21 months. The American Cockroach (as with other pests) is mainly controlled with the use of insecticides. In the current analysis, Propoxur a carbamate insecticide was employed. Carbamate insecticides are derivatives of carbamic acid with the chemical formula of HOC(O)NH2 and elicit paralytic effects on the nervous system with its anticholinesterase action resulting in death. Propoxur appears to be highly effective with cockroaches that display a resistance to organophosphates. Lethal dose 50 (LD50) is a standard measure of toxicology which represents a dose expressed in milligrams (mg) per kilogram (kg) of mass to kill half (50%) of the test subjects. In acute and chronic toxicity the test subjects are exposed to the insecticide and the lower the LD50 the greater the substances’ toxicity. According to relative toxicity substances may be categorised by comparing LD50 levels.

materials and methodologyy

materials and methodologyy

Please refer to the Pharmacology 1 – Range Finding 1 (Page 21) for a complete listing of materials and detailed procedures. Calculations:
Propoxur (purity) = 97.6% (this was the sample provided)
Therefore for an initial stock solution of 25 ml, 1% w/v, amount of Propoxur (97.6% pure) weighed was 0.2561 g. (0.25/0.976 = 0.2561)

REsults

REsults

Table 1: Class results (Group 5/6) Cockroach deaths results as per concentration of propoxur ( % w/v). Control| 0.005| 0.01| 0.02| 0.05| 0.1|
0| 0| 1| 3| 5| 5|
0| 4| 4| 5| 5| 5|
0| 0| 0| 2| 3| 5|
0| 0| 3| 2| 3| 5|
0| 0| 1| 2...
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