The New D-Dimer Assay

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UNIVERSITY OF HULL
MODULE NO: 58372
Laboratory based skills 4

Noel Bacasmas
Student no: 201012397
BSc Applied Biomedical Science
(Part time)

ContentsPage No.

Title and learning objectives.....................................................................3

Introduction.............................................................................................5

Mechanisms of Fibrinolysis.......................................................................6

Different methodologies/Assays to Detect Fibrinolysis...................,,.......12

Venous Thromboembolism; Pathophysiology, Clinical features and Diagnosis.....................................................15 Materials and Methods...........................................................................20

Results.....................................................................................................22

Discussions..............................................................................................23

Conclusion...............................................................................................23

References..............................................................................................25

Appendices.............................................................................................26 LEARNING CONTRACT

TITLE OF LEARNING CONTRACT:
D-dimer HS, a new highly specific quantitative assay for D-dimer testing. LEARNING OUTCOMES:
* Make a logical presentation of the learning contract.
* Research relevant literatures from textbooks, scientific journals, standard operating procedures in the laboratory, manufacturer’s manuals and internet website. * Evaluate and validate the performance of the new test kit D-dimer HS using the ACL TOP analyzer by comparing it with the Hemosil D-dimer assay the current kit SGH is using. * Gain knowledge and understanding about the fibrinolytic system in blood coagulation. * Gain knowledge and understanding about the clinical applications of D-dimer test. * To know the procedure for D-dimer testing in Scarborough General Hospital (SGH). * To establish the differences in the composition of this new D-dimer HS kit with that of Haemosil D-dimer kit that leads to its greater specificity. * Gain practical experience and competence in the use of the ACL TOP analyzer, the automated technology used by SGH for D-dimer testing. * Show understanding of the quality management scheme (Internal quality assurance and the UK-NEQAS) used by SGH to maintain satisfactory standards in D-dimer testing. * To analyze and present results derived from the supervised practical work. * To demonstrate ability to work within professional guidelines, following SOP’s, health and safety protocol and right ethics.

SUPPORTING EVIDENCE:
This learning contract will be fulfilled by a 5000 word document to include the following: * A 500 word introduction describing the scope, the aims and the reason for choosing the topic. * A discussion on the fibrinolytic system in blood coagulation. * A discussion on the different methodologies/assays in D-dimer testing, including their merits and disadvantages as compared to D-dimer HS. * A discussion on the role of D-dimer testing in common clinical scenarios; D-dimer testing in deep venous thrombosis (DVT) and D-dimer testing in pulmonary embolism (PE). * A discussion on the Pathophysiology, clinical features and diagnosis of Venous Thromboembolism (VTE). * Produce a procedure for doing D-dimer testing including sample collection and preparation, the principle of the test and limitations of the test. * Present SOP for the use of the ACL TOP analyzer including its maintenance and quality control schemes. * A statistical analysis and discussion of results obtained by comparing D-dimer levels obtained using the new test kit...
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