The in-Vitro Acetylcholine Antagonism Study of Tomato (Solanum Lycopersicum) Compared to Lagundi (Vitex Negundo L) Using Rat Duodenum

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  • Topic: Asthma, Tomato, Plum tomato
  • Pages : 13 (2932 words )
  • Download(s) : 32
  • Published : February 24, 2013
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Chapter I

Background of the Study:
Bronchial asthma is a chronic relapsing inflammatory disorder with increased responsiveness of tracheobronchial tree to various stimuli, resulting in paroxysmal contraction of bronchial airways. It refers to a condition of subjects with widespread narrowing of the bronchial airways which changes in severity over short periods of time, either spontaneously or under treatment, and is not due to cardiovascular disease According to the CDC, more than 22 million Americans, including 6.5 million children under 18, suffer with asthma today. Allergy clearly plays an important role in many asthma cases but not in all. Although drug-induced bronchospasm occurs most commonly in patients with known asthma, the agents themselves may produce asthma. The best-known of these is aspirin, but several other anti-inflammatory agents have been implicated. 4

Tomatoes are commonly used for cooking. They are rich in lycopene and glutamate which is why they are both healthy and delicious. Although tomatoes are perceived as vegetables, fact is, they are fruits. They are made in different forms such as tomato sauce, tomato juice, tomato soup, sun-dried tomatoes, canned tomatoes and many more. They contain such health benefits, one of which is its ability to treat asthma. So many people suffer from this kind of sickness, and sometimes, it causes an individual’s death. The medication for asthma is expensive, thus we, the researchers, decided to investigate whether the tomato juice extract can be used as a substitute anti-asthma medicine.

Statement of the Problem:
Basically, the problem of the researchers in this study is to determine whether there is any scientific basis for the folkloric claim that Solanum lycopersicum (Plum Tomato) possesses anti-asthma (bronchodilating) property comparable to the herbal standard bronchodilator, which is

Vitex negundo (Lagundi aqueous) extract.

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Objectives:

Specifically, it sought to:

1. Compare the antagonism effect of tomato given in different dose rates with acetylcholine treated duodenum of rats.
2. Determine the linearity effect of increasing dose rates of tomato against supramaximal contracted duodenum.
3. Compare the mean antagonism effect of different dose rates against the supramaximal contracted duodenum.
4. Compute for the ED 50 of the tomato fruit extract.

Significance of the Study:
The study seeks to prove the folkloric claim of tomato fruits or effective bronchodilator. When proven successful, this study will benefit the people who cannot afford to buy expensive anti-asthma medicines since tomatoes are abundant in the locality. Furthermore, there will be now more choices of indigenous plants that can relieve asthma, aside from the Indian Mango and Lagundi leaves.

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Scope and Limitation:
This study is limited to the use of the tomato fruit extract of the “Plum” tomato variety. It is also limited to the determination of pharmacologic effect (bioassay) of a drug, an in-vitro bioassay type of study of tomato fruit extract using the duodenum of five (5) ICR Albino rats.

Hypotheses:
Null: There is no significant difference in the bronchodilating of tomato fruit extract and Lagundi.
Alternative: The tomato fruit extract is a better bronchodilator compared to Lagundi.

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Chapter III
Methodology
Materials:

1. 2 kilos of tomato fruits
2. Acetylcholine concentration
3. Physiologic saline solution – Tyrode Solution consisting of solutions A and B

A :
Sodium Chloride or NaCl = 80 gms
Calcium Chloride or CaCl = 2 gms
Potassium Chloride or KCl = 2gms
Magnesium Chloride or MgCl² = 0.1gms

B :
Sodium Carbonate or NaHCO³ = 10gms
Sodium Phosphate or NaH2PO4 = 0.5gms

4. 8 liters of distilled water
5. 5 isolated rat duodenum tissue

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Apparatus:
1. Muscle warmer – container of the tissue (broken fluorescent bulb) 2....
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