The Efficacy of Takip-Kohol (Centalla Asiatica) Leaves Extract as an Antibacterial Agent Against Escherichia Coli, Staphylococcus Aureus and Salmonella Enteritidis.

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  • Topic: Bacteria, Microbiology, Escherichia coli
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  • Published : March 12, 2013
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THE EFFICACY OF TAKIP-KOHOL (Centella asiatica) LEAVES EXTRACT AS ANTIBACTERIAL AGENT AGAINST Escherichia coli, Salmonella enteritidis AND Staphylococcus aureus

CLUSTER 1 LIFE SCIENCE TEAM CATEGORY

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ABSTRACT THE EFFICACY OF TAKIP-KOHOL AGAINST (Centella Escherichia asiatica) coli, LEAVES Salmonella EXTRACT enteritidis AS AND

ANTIBACTERIAL Staphylococcus aureus

AGENT

This research study aims to discover the efficacy of Takip-kohol leaves extract as antibacterial agent against Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. The study was conducted to produce an antibacterial agent that is effective, for the wide spread of diseases in several countries nowadays. There were different procedures used in the experiment: culturing the test organisms, extraction of Takip-kohol leaves (pure and boiled extracts) to determine the significant difference between the two and Kirby-Bauer Method. The study conducted was proven to be effective as antibacterial agent. There is a significant difference between pure and boiled Takip-kohol leaves extract. Pure extract of Takip-kohol leaves is proven to be effective as antibacterial agent against Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. Boiled extract is proven to be effective as antibacterial agent against Salmonella enteritidis and Staphylococcus aureus and not on Escherichia coli.

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ACKNOWLEDGMENT

We, the fourth year students would like to express our deepest and sincerest gratitude to the following people who in one-way have given help to make this piece of work possible. Our Research Adviser MRS. KARLA MAE LOPEZ for giving us suggestions for the process and improvement of the research work and for understanding our every shortcoming. We would like to thank MRS. MELANY BOTE for giving us references and for the developments with regards to our project. Also to DR. ALISHA REYES for sharing us her knowledge and skills to make the research work more efficient. Next to TITA DANG DOMINGO for giving her full efforts in helping us with our experiment and for lending us the materials to us, to make the experimentation possible. To ATE AMICKA MADRIAGA for giving suggestions in doing the project. Of course, we would like to give a warm thank you, to our parents for being an answered prayer, the success of this project is impossible without you. To our classmates (IV-DIAMOND) for helping us, enlightening us and giving moral support, to continue our research although we feel hopeless, that we are unable to finish this, due to lack of time. And above all, the LORD our GOD Almighty for giving us patience, the critical thinking, through the entire process, without HIM we will never succeed, the success of this work is for the Glory of HIM.

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Table of Contents Title Abstract Acknowledgment Table of Contents List of Tables List of Figures I. The Problem and Its Background Introduction Background of the Study Statement of the Problem General Objective Specific Objectives Hypotheses Significance of the Study Review of Related Literature Scope and Limitations Definition of Terms II. III. Methodology Results and Discussion 26 28 1 2 3 4 5 6 7 7 8 10 10 10 10 11 11 14 15 16 23

IV. Summary of the Findings, Conclusion and Recommendations V. Bibliography

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List of Tables Table 1. Results of antibacterial test of Takip-kohol leaves extract against Escherichia coli. Table 2. Measurements of inhibition of Takip-kohol leaves extract against Escherichia coli

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Table 3. . Results of antibacterial test of Takip-kohol leaves extract against Salmonella enteritidis. Table 4. Measurements of inhibition of Takip-kohol leaves extract against Salmonella enteritidis. Table 5. Results of antibacterial test of Takip-kohol leaves extract against Staphylococcus aureus. Table 6. Measurements of inhibition of Takip-kohol leaves extract against Staphylococcus aureus. Table 7. Final results...
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