Preview

The Efficacy of Takip-Kohol (Centalla Asiatica) Leaves Extract as an Antibacterial Agent Against Escherichia Coli, Staphylococcus Aureus and Salmonella Enteritidis.

Powerful Essays
Open Document
Open Document
4852 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
The Efficacy of Takip-Kohol (Centalla Asiatica) Leaves Extract as an Antibacterial Agent Against Escherichia Coli, Staphylococcus Aureus and Salmonella Enteritidis.
THE EFFICACY OF TAKIP-KOHOL (Centella asiatica) LEAVES EXTRACT AS ANTIBACTERIAL AGENT AGAINST Escherichia coli, Salmonella enteritidis AND Staphylococcus aureus

CLUSTER 1 LIFE SCIENCE TEAM CATEGORY

1|Page28

ABSTRACT THE EFFICACY OF TAKIP-KOHOL AGAINST (Centella Escherichia asiatica) coli, LEAVES Salmonella EXTRACT enteritidis AS AND

ANTIBACTERIAL Staphylococcus aureus

AGENT

This research study aims to discover the efficacy of Takip-kohol leaves extract as antibacterial agent against Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. The study was conducted to produce an antibacterial agent that is effective, for the wide spread of diseases in several countries nowadays. There were different procedures used in the experiment: culturing the test organisms, extraction of Takip-kohol leaves (pure and boiled extracts) to determine the significant difference between the two and Kirby-Bauer Method. The study conducted was proven to be effective as antibacterial agent. There is a significant difference between pure and boiled Takip-kohol leaves extract. Pure extract of Takip-kohol leaves is proven to be effective as antibacterial agent against Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. Boiled extract is proven to be effective as antibacterial agent against Salmonella enteritidis and Staphylococcus aureus and not on Escherichia coli.

2|Page28

ACKNOWLEDGMENT

We, the fourth year students would like to express our deepest and sincerest gratitude to the following people who in one-way have given help to make this piece of work possible. Our Research Adviser MRS. KARLA MAE LOPEZ for giving us suggestions for the process and improvement of the research work and for understanding our every shortcoming. We would like to thank MRS. MELANY BOTE for giving us references and for the developments with regards to our project. Also to DR. ALISHA REYES for sharing us her knowledge and skills to make the research work more efficient. Next

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Escherichia coli is a rod-shaped Gram-negative bacteria from the Enterobacteriaceae family, which owns the usual flora and can be be as pathogenic Human IN DUE can be cause diarrhea. The rational NOT USE ON antibiotics Infectious diseases can be causing the occurrence of resistance so that the necessary alternative to using Active Ingredients antimicrobial From medicinal plants. Turmeric (Curcuma domestica Val.) It is a prayer One Crop Growing in Indonesia That can be used as a medicinal plant. BECAUSE containing Active Compounds form of curcumin, flavonoids, alkaloids, tannins and Yang has a function as an antibacterial function. Singer study conducted to determine ACTIVITIES review antibacterial extract turmeric (Curcuma domestica Val.) Against the growth of Escherichia coli isolates from diarrhea patients in Hospital Muhammadiyah Palembang.…

    • 284 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Plants such as Glycyrrhiza glabra, Allium sativum, Aloe vera, Physalis angulata, Annona senegalensis, Dryopteris crassirhizoma, Quercus infectoria, Englerophytum magalismontanum, Euclea natalensis, Solanum panduriforme, Rosmarinus officinalis Linn., Baeckea frutescens, and Parinari curatellifolia were demonstrated to have antibacterial activity against S. mutans (Bachrach et al., 2011; Ban et al., 2012; Fani and Kohanteb, 2012; Hwang et al., 2004; More et al., 2008). In addition, substances such as linoleic, linolenic, oleanolic, betulinic acids, betulin, and beta-sitosterol glucoside, among others, were reported to suppress adherence of S. mutans in-vitro (Wu,…

    • 564 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Oregano is a traditional herb that is known for its natural immune support. Its oil contains carvacrol, a monoterpene phenol that serves as its primary germ-killing ingredient, and it provides many of the health benefits of the oregano. It also contains thymol, a powerful substance which is also a monoterpene phenol and is a natural fungicide with antiseptic properties. Esters are also strong antifungal agents, and two of these are abundant in oregano oil, namely linalyl acetate and geranyl acetate. Together, these substances make oregano oil a great natural alternative to treat infections. These substances also made the researchers see the potential of oregano oil as a possible…

    • 798 Words
    • 3 Pages
    Good Essays
  • Better Essays

    May. J., Chan. C. H., King. A., Williams. L., French. G. L. (1999) Time–kill studies of tea tree oils on clinical isolates. Journal of Antimicrobial Chemotherapy. 45, Issue5: 639-643…

    • 1153 Words
    • 5 Pages
    Better Essays
  • Powerful Essays

    Herbs and spices have been used for many years for their health benefits and antimicrobial properties (Lai & Roy, 2004). Cilantro, an herb found in many ethnic food dishes, has been shown to have an antibacterial effect on Salmonella choleraesuis due to the compound dodecanal found in the leaves. Dodecanal was found to be twice as potent as the antibiotic gentamicin that is commonly prescribed for Salmonella infections (Kubo, Fujita, Kubo, Nihei, & Ogura, 2004). The antimicrobial properties of cilantro have also been demonstrated against the bacteria’s Staphylococcus aureus, Bacillus cereus, Escherichia coli (Marsh & Arriola, 2009). We decided to conduct our own…

    • 2091 Words
    • 9 Pages
    Powerful Essays
  • Powerful Essays

    The results show that tea tree and eucalyptus have about the same antibacterial activity as the positive control (ampicillin) used in this experiment. Oregano and colloidal silver’s zone of inhibition were closer to h2O, which concludes that at the amount used for both there is no antibacterial…

    • 939 Words
    • 4 Pages
    Powerful Essays
  • Powerful Essays

    INVESTIGATING THE EFFECT OF A LOCAL HERB- P. AMARUS (SCHUM. AND THONN.) ON ENTEROPATHOGENIC STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI. BY ETTA, Hannah Edim, ELOMA, Nnanke, OKON, Essien Archibong and OFFOR, Ubana BIOLOGICAL SCIENCE DEPARTMENT, CROSS RIVER UNIVERSITY OF TECHNOLOGY , CALABAR, CROSS RIVER STATE, NIGERIA. Abstract Objectives: Entero-pathogenic Staphylococcus aureus (EPSA) and Escherica coli (EPEC) were treated with a methanol extract of Phyllanthus amarus, in vitro, to investigate the possible effects of the herb on these organisms. Zones of Inhibition (ZI) of the extract on EPSA and EPEC cultures and the Minimum Inhibitory Concentrations (MIC) of the methanolic extracts were determined using standard procedures. EPEC showed mean ZI of 17.7 ± 2.56, while the mean ZI for EPSA was 14.79 ± 3.87. Statistical analyses showed positive correlation of 0.56 at P≤0.1 between the plant extract concentrations and EPEC ZI and 0.75 at P ≤0.1 for the EPSA. At the concentrations administered, the extract was active against both EPSA and EPEC isolates. These results present the herb, P. amarus as a possible biotechnological tool in the field of herbal medicine for the control of antibiotic-resistant, entero-pathogenic S. aureus and E. coli infections. Keywords: Entero-pathogenic bacteria, Phyllanthus amarus, Staphylococcus aureus and Escherica coli. Introduction The use of herbs and herbal derivatives in Traditional Medicine Practices (TMP), as a biotechnological tool, has come to stay, not just in Nigeria but in most tropical regions of the world where there are vast, dense, evergreen forests that nature has presented to humanity as a gift of a therapeutic workshop with a wide variety of medicinal plants. With the recent advances in contemporary alternative medicine (CAM) and the emergence of multi resistant strains of microbials, it…

    • 2287 Words
    • 10 Pages
    Powerful Essays
  • Powerful Essays

    This study was conducted to determine the potential of Impatiens balsamina Linn. (Kamantigue) Flower Extract as an antifungal Agent for Candida albicans and Trichophyton mentagrophytes…

    • 8646 Words
    • 35 Pages
    Powerful Essays
  • Good Essays

    Associate Professor and Research scholars, NBKR Medicinal Plant Research Centre, Vidyanagar – 524413, SPSR Nellore Dist, Andhra Pradesh, India.…

    • 662 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Cassia fistula Linn. (Leguminosae) is a very common plant and is widely known for its medicinal properties. In the Indian literature, this plant has been described to be useful against skin diseases, liver troubles, tuberculous glands and its use in the treatment of rheumatism, hematemesis, pruritus, leucoderma, and diabetes.[1,2] Besides, it has been found to exhibit anti-inflammatory and hypoglycemic activity and widely used as a mild laxative suitable for children and pregnant women.[3] Several reports are present on hepatoprotective,[4] antifertility,[5] and antioxidant properties of C. fistula.[6,7] Some studies have also been done on antimicrobial activity of C. fistula flower and seed along with some other Indian medicinal…

    • 1301 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    against bacterial species and fungal species have shown that the antibacterial effect was higher against fungal species. In a study by Karim, Asma and Kamal et al., (2016) in vitro antimicrobial activity of Sudanese Cucumis melo L. fixed oil was investigated. Agar cup diffusion assay was performed against two fungal species (Candida albicans and Aspergillus niger), two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Ampicillin, gentamycin and clotrimazole were used as positive controls. Antimicrobial activity was shown by all the species except E.coli and P. aeruginosa. Oil showed a highest activity against Candida albicans with an inhibition zone of 19mm and a partial activity of 12mm against Staphylococcus aureus. A similar study was done in Bangladesh on the isolation, physico-chemical characterization and microbial activities of melon (Cucumis melo) seed oil (Ahamed et al., 2014). The antimicrobial activity was studied on four bacterial species (Staphylococcus aureus, Escherichia coli, Salmonella typhi and Bacillus cereus) and four fungal species (Aspergillus funiculosus, Fusarium equiseti, Curvularia lunata and Alternaria alternate). Oil samples of 1% and 10% showed activity against all test microbes except for Curvularia lunata. From bacterial species, highest inhibition zone (19 mm) was observed…

    • 1611 Words
    • 7 Pages
    Good Essays
  • Satisfactory Essays

    Noasdfgdfafsdasdasd

    • 953 Words
    • 8 Pages

    An Antimicrobial Ointment Manufactured from Kamantigue (Impatiens balsamina) Leaves Extract A Comparison with Miconazole Nitrate…

    • 953 Words
    • 8 Pages
    Satisfactory Essays
  • Good Essays

    Alululuba

    • 298 Words
    • 2 Pages

    A Study on Feasibility of Sambong (Blumea balsamifera) Leaves, Onion (Allium cepa) Bulb, and Takip Kuhol (Centella asiatica) Leaves Extract as Antibacterial of Salmonella typhimurium, Staphylococcus aureus and Pseudomonas aeruginosa…

    • 298 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Http

    • 3856 Words
    • 15 Pages

    Malnutrition and diseases caused by it are one of the worldwide problems we are facing. Malnutrition is caused by lack of intake of nutrients which stops the body functioning as it should be. Malnourished people- especially children, experience developmental delays, weight-loss and illness. Malnourishment can greatly compromise a child’s immune system, making them more susceptible to infectious diseases. Particularly in institutions where there are poor sanitary practices, children are vulnerable to infections from other children or caregivers.…

    • 3856 Words
    • 15 Pages
    Powerful Essays
  • Powerful Essays

    Agar well diffusion method was used to assess the activity against the bacteria by measuring zone of inhibition. Inhibiting concentrations used for all the extracts was 500 mg/ml. Methanolic extract of C. album was found to be most active among all other extracts and inhibits Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Escherichia coli. The extract of a mixture of solvents was also found to be effective in inhibiting Staphylococcus aureus. Methanolic extract of C. album was found to give a most clear zone of inhibition. The antibacterial activity is attributed to the phenolic content present in the sample extract. The samples which had higher phenolic content were found to be better at inhibiting the growth of bacteria, hence giving zone of clearance of greater diameter. The zone of clearance or zone of inhibition of different extracts of C. album against various bacterial growths is listed in the Table…

    • 1737 Words
    • 7 Pages
    Powerful Essays