Impatiens Balsamina Linn. (Kamantigue) Flower Extract: a Potential Antifungal Agent for Candida Albicans and Trichophyton Mentagrophytes

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  • Topic: Candidiasis, Candida albicans, Yeast
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Impatiens balsamina Linn. (Kamantigue) Flower Extract:
A Potential Antifungal Agent for Candida albicans and Trichophyton mentagrophytes

A
Thesis
Presented to the
Faculty of the College of Medical Technology
Unciano Colleges Inc-Antipolo

In Partial Fulfillment
Of the Requirements for the Degree
Bachelor of Science in Medical Technology

By:
Bernardo, Reynalyn
Cruz, Nor Rizsellito
Lagman, Joyce Anne
Leonor, Zheidi Ann Mari
Samson, Danica
November 2012

ABSTRACT

This study was conducted to determine the potential of Impatiens balsamina Linn. (Kamantigue) Flower Extract as an antifungal Agent for Candida albicans and Trichophyton mentagrophytes This study employed the experimental research method. The experimental group method in this study consisted of six (6) petriplates. Three (3) petriplates for Candida albicans, and three (3) petriplates for Trichophyton mentagrophytes. Each petriplates were treated with 80% ethanol, Nystatin and Impatiens balsamina Linn.. flower extract with 80% ethanol that were all soaked in Whattman No.1 Filter paper disk. The experimental group was treated with 100% level of concentration of Impatiens balsamina Linn. flower extract. After 18 to 24 hours of incubation at 37°C, the zones of inhibition after the exposure of Impatiens balsamina Linn. to the Candida albicans and Trichophyton mentagrophytes were measured and compared with the zone of inhibition of Nystatin and 80% Ethanol respectively. Based on the data gathered, the mean of the experimental group’s zone of inhibition after its exposure to Candida albicans was 6.3333 mm; the mean of the positive control was 10.3333 mm and the mean of the negative control was 8.5. The value of significance at 95% level of confidence was 0.402. On the other hand, the mean of the experimental group’s zone of inhibition after its exposure to Trichophyton mentagrophytes was 10.3333 mm; the mean of the positive control was 12 mm and the mean of the negative control was 10.3333mm. The value of significance at 95% level of confidence was 0.302. It was therefore concluded since there was no significant difference in the zone of inhibition of the experimental group after its exposure to Candida albicans and Trichophyton mentagrophytes compared with the zone of inhibition of the control groups, the Impatiens balsamina Linn. flower extract has no possible antifungal property.

TABLE OF CONTENTS

Title Page ……………………………………………………………………………………….... i Approval Sheet ………………………………………………………………………………….. ii Acknowledgement ……………………………………………………………………………... iii Abstract ………………………………………………………………………………………….. iv Contents ……………………………………………………………………………………….. v List of Tables ………………………………………………………………………………….... vi Lists of Plates …………………………………………………………………………………... vii

CHAPTER I
Introduction …………………………………………………………………………….. 1 Statement of the Problem ……………………………………………………………. 4 Hypotheses …………………………………………………………………………….. 4 Significance of the Study ……………………………………………………………... 5 Definition of Terms ……………………………………………………………………. 6 Scope and Limitations ………………………………………………………………… 7

CHAPTER II
REVIEW OF RELATED OF LITERATURE ---------------------------------
JUSTIFICATION OF THE STUDY--------------------------------------------

CHAPTER III
RESEARCH DESIGN-------------------------------------------------------------
TEST ORGANISM-----------------------------------------------------------------
GATHERING OF DATA----------------------------------------------------------
COLLECTION OF DATA---------------------------------------------------------
COLLECTION OF THE PLANT SAMPLE-----------------------------------
PREPARATION OF THE PLANT SAMPLE---------------------------------
EXTRACTION PROCEDURE--------------------------------------------------

STANDARD INGREDIENTS IN
SABOURAUD DEXTROSE AGAR-----------------------------------
PREPARING SABOURAUD DEXTROSE AGAR-------------------------
PREPARATION OF...
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