The Effect of Pilocarpine and Tropicamide on Human

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  • Topic: Pupil, Eye, Atropine
  • Pages : 3 (1012 words )
  • Download(s) : 1190
  • Published : February 27, 2011
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1. The Effect of Pilocarpine and Tropicamide on Human’s Pupil

2. Throughout the experiment, we have learned how to measure the pupil diameter using a pupillometer, how to measure the near point of accommodation using a accommodometer and how to demonstrate the pupillary light reflexes by shining the light on the pupil. Besides that, we have learned the effect of 1% tropicamide and 2% pilocarpine on the human’s pupil and how the drugs will affect the pupil diameter, near point of accommodation and the pupillary light reflexes.

3. Before one drop of 1% tropicamide was instilled into the pouch of subject A’s left eye, subject A was required to carry out the experiment for 15 minutes without the influence of the drug to obtain four baseline readings. Each baseline reading was taken at the interval of 5 minutes. The baseline readings obtained from subject A remained constant at 2.0mm from 0 to 15 minutes which means that the pupil diameter of subject A’s left eye remained constant at 2.0mm from 0 to 15 minutes without the influence of 1% tropicamide. At 15 minutes, one drop of 1% tropicamide was instilled into the pouch if subject A’s left eye. From 15 to 35 minutes, the pupil diameter of subject A’s left eye increased steadily from 2.0mm to 4.5mm. After 35 minutes, the pupil diameter of subject A’s left eye reached a plateau and the pupil diameter remained constant at 5.5mm until 90th minute. As for the right eye of subject A, subject A was required to take four baseline readings for the 1st 15 minutes at the interval of 5 minutes before one drop of 0.9% saline was instilled into the pouch of subject A’s right eye. The baseline readings obtained by subject A remained constant at 2.5mm throughout the 1st 15 minutes. However, the pupil diameter of subject A’s right eye did not change after 15minutes and remained constant at 2.5mm throughout the experiment. As for subject B, before one drop of 2% pilocarpine was instilled into the pouch of subject B’s left...
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