Starch Lab Report

There was a controlled and an experimental substance. The controlled substance was the one with starch in the dialysis bag, and the experimental substance was the one with starch and amylase in the dialysis bag. Both had the same solvent outside of the bag (Lugols and Distilled Water). The color change differed from inside and outside the bag as time went on, and at the end of the 45 minutes, the two bags had changed colors.
The solute in the controlled substance had a darker color to it, with it looking a majority blue/black. The solute in the experimental substance had a gray color to it. The reason for the color change, was mostly because of the lugols. Lugols is an indicator that turns black/blue whenever there is starch. The reason the controlled substance had turned black was because some of the lugols was able to diffuse into the dialysis bag. The Lugols (KI3) was able to diffuse through the membrane, because the molecules were small enough to pass through the semipermeable membrane and into the starch. The outside of the dialysis bag, with the Lugols and distilled
Amylase is an enzyme that digests carbohydrates and breaks down the hydrolysis of starch into sugars. The starch molecules were not able to cross the membrane of the bag, because they were too large. However, when broken down by the amylase in the experimental substance, the starch molecules that were turned into sugar were able to cross the membrane, giving us a glucose level in the distilled water and Lugols.
Since the glucose went from a higher concentration to a lower concentration, that is why the molecular transport was diffusion. The passive transport gave us a larger amount of glucose in the solute and a smaller amount in the solvent. Osmosis also occurred, because the water molecules were able to get past the permeable membrane giving us a larger volume in the dialysis bag, then what we started