Solute Concentration of Potatoes

Nov 19/12 Solute Concentration of Potatoes Block 2-2

Purpose: To determine the concentration of solute in the potato’s cytoplasm by measuring the change in mass after the process of osmosis.

Materials and Equipment: Refer to Biology 12 Lab Manual – Investigation 13

Procedure: Refer to Biology 12 Lab Manual – Investigation 13

Data and Observations:

The Potatoes change in mass after the process of Osmosis Test Tube # | Concentration of Sucrose Solution (mol/L) | Initial mass (g) | Final mass (g) | Change in mass (g) | Percentage change in mass (%) | 1 | 1.0 | 5.12 | 3.63 | -1.49 | 29.0% | 2 | 0.9 | 6.01 | 4.30 | -1.71 | 28.0% | 3 | 0.8 | 6.50 | 4.82 | -1.68 | 26.0% | 4 | 0.7 | 5.92 | 4.58 | -1.34 | 23.0% | 5 | 0.6 | 6.10 | 4.87 | -1.23 | 20.0% | 6 | 0.5 | 6.02 | 5.09 | -0.93 | 15.0% | 7 | 0.4 | 6.20 | 5.67 | -0.53 | 9.0% | 8 | 0.3 | 5.54 | 5.53 | -0.01 | 0.18% | 9 | 0.2 | 6.40 | 6.59 | 0.19 | 3.0% | 10 | 0.1 | 5.58 | 6.00 | 0.42 | 7.5% | 11 | 0.0 | 5.56 | 6.15 | 0.59 | 11.0% |

Questions:

1) As the Concentration of the Sucrose Solution increases, the more the potato’s mass decreases. This is due to the solution being hypotonic. So, as the solute concentration gets higher, the potato’s water concentration will get