Preview

Screening of Chemical Composition of Essential Oil

Powerful Essays
Open Document
Open Document
2889 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Screening of Chemical Composition of Essential Oil
Journal of Medicinal Plants Research Vol. 5(10), pp. 2035-2040, 18 May, 2011 Available online at http://www.academicjournals.org/JMPR ISSN 1996-0875 ©2011 Academic Journals

Full Length Research Paper

Screening of chemical composition of essential oil, mineral elements and antioxidant activity in Pulicaria Undulata (L.) C. A. Mey from Iran
Mehdi Ravandeh1, Jafar Valizadeh2*, Meissam Noroozifar1 and Mozhgan Khorasani-Motlagh1
1

Department of Chemistry, University of Sistan and Baluchestan, Zahedan, P. O. Box 98155-987, Iran. 2 Department of Biology, University of Sistan and Baluchestan, Zahedan, P. O. Box 98155-987, Iran.
Accepted 17 March, 2011

Pulicaria undulata (L.) C.A. Mey is a medicinal plant used to treat inflammation. It is also an insect repellent and herbal tea. In this study, concentration of macro minerals (that is, Ca, K and Mg) and micro minerals (major and trace elements) (that is, Fe, Cu, Na and Mn) and P, were estimated in Pulicaria undulata from the Sistan and Baluchestan province. Sample preparation using microwave digestion was used for mineralization of this plant. Atomic absorption spectrophotometry (AAS) and UV-Visible spectrophotometry were used to quantify metal levels and Phosphorus, respectively. Essential oil from aerial parts of P. undulata was obtained by hydrodistillation. The chemical constituents of essential oil of aerial parts were analyzed by GC-FID and GC/MS techniques. The main components identified in the oil were 4-Terpineole (20.12%), alpha terpinene (4.02%), gamma terpinene (7.00%), cis-sabinene hydrate (8.29%), Linalool (5.60%), 1S-cis-calamenene (13.37%) and Junipene (8.66%). In addition, antioxidant activity of ethanolic extract was evaluated by 1,1-diphenyl-2picrylhydrazyl (DPPH) radical scavenging method. Key words: Macro and micro minerals, microwave digestion, Pulicaria undulata, antioxidant activity. INTRODUCTION Essential oils are volatile, natural, complex compounds characterized by a strong odour and



References: Adams RP (2007). Identification of Essential Oil Components by Gas Chromatography/ Mass Spectrometry. 4th Ed. Allured Publishing Corporation, Carol Stream, Illinois, USA. Al RA (1987). Flora of Kuwait. Vol 2. Alden press, Oxford. Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008). Biological effects of essential oils. Food Chem. Toxicol., 46: 446-475. Boulos L (2002). Flora of Egypt. Vol 3. Al-Hadara publishing. Cairo, Egypt. Brand WW, Cuvelier ME, Berset C (1995). Use of a free radical method to evaluate antioxidant activity. Food Sci. Technol., 28: 25–30. EL KHH, Ahmed AH, Mohammed AS, Yahia AAM, EL TIH, Ali AA (1998). Antibacterial properties of essential oils from Nigella sativa seeds, Cymbopogon citratus leaves and Pulicaria undulata aerial parts. Fitoterapia. LXIX, No. m1: 77-78. EL KHH, Yousif MO, Ahmed OI, Sabir SS (2009). Phytochemical analysis of the essential oil from aerial parts of pulicaria undulata (L.) Kostel from Sudan. Ethanobotanical Leaflets, 13: 467-471. Mertz W (1982). Trace minerals and atherosclerosis. Food Proc., 41(11): 2807-2812. Mossa JS, Hifnawy MS, AL YMA, AL MIA, Mekkawi AG (1987). GC/MS analysis of essential oils of Pulicaria arabica and P. undulata. Pharm. Biol., 25(2): 113-119. Nematollahi F, Rustaiyan A, Larijani K, Nadimi M, Masoudi S (2006). Essential Oil Composition of Artemisia biennis Willd. and Pulicaria undulata (L.) C.A. Mey., Two Compositae Herbs Growing Wild in Iran. J. Essent. Oil Res., Ozcan M (2004). Mineral contents of some plants used as condiments in Turkey. Food Chem., 84: 737-740. Ross SA, El SKA, El SMA, Hamann MT, Abdel HOB, Ahmed AF, Ahmed MM (1997). Phytochemical Analysis of Geigeria alata and Francoeuria crispa essential oils. Planta Med., 63: 479–482. Sanchez CCP, Dewey PJS, Aguirre A, Lara JJ, Vaca R, Leondela BP, Ortiz M, Escamilla I, James WPT (1998). The mineral content of Mexican fruits and vegetables. J. Food Compost. Anal., 11: 340-356. Soares JR, Dinis TCP, Cunha AP, Almeida LM (1997). Antioxidant activity of some extracts of Thymus zygis. Free Rad. Res., 26: 469– 478. Stavri M, Mathew KT, Gordon A , Steven D, Shnyder R, Falconer A, 2040 J. Med. Plant. Res. Gibbons S (2008). Guaianolide sesquiterpenes from Pulicaria crispa (Forssk.) Oliv. Phytochem., 69: 1915-1918. Suhaj M (2006). Spice antioxidants isolation and their antiradical activity. J. Food Compost. Anal., 19: 531-537. Tadhani MB, Petal VH, Subhash R (2007). In vitro antioxidants activities of Stevia rebaudiana leaves and callus. J. Food Compost. Anal., 20: 323-329.

You May Also Find These Documents Helpful

  • Better Essays

    The purpose of this lab was to obtain a pure sample of clove oil from ground cloves using steam distillation, perform infrared spectroscopy on the sample, and analyze the IR to identify the major constituent in the clove oil. The first day the clove oil was removed from the ground cloves into an aqueous solution then removed from the water using DCM. The sample was left in a sealed container for one week and then isolated by drying the sample and removing the DCM. After the clove oil was isolated it was analyzed using IR spectroscopy.…

    • 1121 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Returning back to clove oil, which is used in this experiment, this oil is extracted from a small evergreen tree called Syzygium aromaticum that is found in Indonesia, Madagascar, and Zanzibar. Clove oil consists of an essential oil which is a volatile mixture of water-insoluble components that releases an odor and other characteristic of the plant. In this experiment we abstracted the major component of clove oil by codistillation with water through steam distillation and dichloromethane extractions. Also, IR spectrometry was used to identify the unknown constituent. An IR spectrum shows the characteristic…

    • 938 Words
    • 4 Pages
    Good Essays
  • Good Essays

    The use of essential oils began in the nineteenth century being used as flavour and aroma enhancers and quickly became popular. Since then they have become widespread over several industries pharmaceutical, cosmetic, medicinal and in food. There are 300 of the 3,000 different essential oils known being used commercially. As the synthetic preservatives became widely used because of their cheap production and relentless antimicrobial activity the use of essential oils as preservatives became less common and less studied. Nowadays synthetic preservatives such as parabens are frowned upon their use and products containing them will not be bought for fear of their health issues. The over use of antibiotics has led to microbial resistance against…

    • 240 Words
    • 1 Page
    Good Essays
  • Good Essays

    oils found that the oils isolated for 2 and 3 h were stronger free radical scavengers, at the same time as with the thiobarbituric acid reactive substances (TBARS) way, the highest antioxidant values had been acquired in the oils isolated for 30 min, 2 and 3 h. hydroxyl radical scavenging and lipoxygenase activity assays showed the satisfactory outcomes with oils isolated for 1 and 3 h. with the deoxyribose way, sage oils at concentrations <1000 mg L(-1) confirmed higher interest than mannitol. The important oil of S. Officinalis proved very weak antimicrobial hobby (Miguel et al.,…

    • 759 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Australian tea tree is the great source of aromatherapy essential oils. This tree has numerous health benefits and effects than any other trees. Every parts of this tree are beneficial to any disease of the human. The leaves of this tree cure the skin diseases of the human and give most attractive skin. In the first aid box, this aromatherapy essential oil has a vital role to cure numerous injuries and diseases. Many people struggle to breathe due to their cold, cough or any sinus problems. These aromatherapy essential oils are very useful in making the people breathing easier and give the peaceful sleep. People can inhale the fragrant essential oil that helps them to open up their air ways from congestion. Aromatherapy essential oils are volatile in nature and it is also known as hydrophobic liquid that is created naturally. Some of the products of essential oils in the market are rose oils, eucalyptus essential oils, and lavender…

    • 450 Words
    • 2 Pages
    Good Essays
  • Good Essays

    The history of essential oils goes all the way back to the time of the bible. Essential oils that are mentioned in the bible are cedarwood, frankincense, fir, cinnamon, myrrh, myrtle and spikenard. Essential oils can be found in different types of plants. For example, frankincense can be found in a frankincense tree. Today’s recipes/blends for essential oils are a direct result of biblica references. People around the world use essential oils every day to help with illnesses, stress and other uses such as fragrance bathing products, incense, perfumes, and cosmetics.…

    • 274 Words
    • 2 Pages
    Good Essays
  • Good Essays

    An Essential oil is the fluids from a plant in which it take on the odor and/or taste of the plant one extracts it from. Essential oils have been used through out history dating back to the bible, but had gone cold until 1928 when a French chemist by the name of Rene-Maurice Gattefosse had accidentally burned his hand severely while working. He plunged his hand into the nearest container which happened to be lavender oil instead of Water. To his surprise, his hand did not have any burn scars to be seen. This “ Re-discovery” has opened doors to thousands of uses of essential oils.…

    • 478 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Isolation of Eugenol

    • 559 Words
    • 3 Pages

    In this experiment, the essential oil eugenol (the main component of oil of cloves) will be isolated from whole cloves using the technique of simple distillation, which is often used to isolate liquid natural products from plants. The mixture of whole cloves and distilled water will then be heated to boiling and the distillate (a eugenol/water mixture) will be collected. The eugenol will then be separated from the water by extraction with pentane. The pentane solutions will then be dried over magnesium sulfate, remove the magnesium sulfate by vacuum filtration and remove the pentane from the filtrate by evaporation on a water bath in the hood. The percent recovery from cloves will be determined and the product will be analyzed by transmission infrared spectroscopy (IR).…

    • 559 Words
    • 3 Pages
    Good Essays
  • Better Essays

    M. Officinalis Lab Report

    • 1103 Words
    • 5 Pages

    Samples of dried aerial parts (300g each sample) of M. officinalis were hydrodistilled for 4 h in a modified Clevenger-type apparatus to obtain the volatile constituents. The essential oil of M. officinalis was analysed by GC on a Trace GC ULTRA with FID detector gas chromatograph equipped with a column (30 m…

    • 1103 Words
    • 5 Pages
    Better Essays
  • Good Essays

    According to Afolayan et al (2007), phenolic content of plant extracts could be responsible for their free radical scavenging activity and studies on fruits and vegetables also show a relationship between phenolic content and antioxidant activities (Omken et al., 2009). The antioxidant potential of polyphenols arises due to their high reactivity as hydrogen or electron donors, which can stabilize and delocalize the unpaired electron and chelate metal ions (Rice-Evans et al., 1997). In agreement with the above report, the ethyl acetate fraction (ABE) exhibited the highest phenolic content when compared with lyophilized aqueous extract, petroleum ether, butanol and aqueous fractions. These results suggested that the phenolic compounds significantly contributed to the antioxidant capacity of the Averrhoa bilimbi…

    • 968 Words
    • 4 Pages
    Good Essays
  • Good Essays

    may have antibacterial, antioxidant and anticancer properties. In the present study, phenolic composition, antibacterial activity and…

    • 1754 Words
    • 8 Pages
    Good Essays
  • Good Essays

    The ABTS values of the antioxidant extracts are presented in Table 2. Mahua extracts from different extraction solvents differed significantly (P < 0.05) in their ABTS values. ABTS radical scavenging ability of samples can be ranked as acetone > methenol > meth – ethanol > water > ethenol fraction. The ABTS values of mahua flower ranged from 12.243±0.745 to 74.18±1.155 µmol Trolox/g fw ; unripe fruit from 20.196±0.332 to 59.1±0.1 µmol Trolox/g fw ; and ripe fruit from 13.64± 0.529 to 45.22 ± 1.105 µmol Trolox/g fw . The results suggested that 50 % acetone was the best solvent for extracting ABTS antioxidant assay for mahua flower, unripe and ripe…

    • 1312 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Fenugreek Case Study

    • 1823 Words
    • 8 Pages

    Fenugreek (Trigonella foenum graceum L.), an indigenous spice crop of India has been widely used in traditional medicine for treating various kinds of diseases. In this work, 10 genotypes of T. foenum-graceum were evaluated for their diosgenin content and productivity, ascorbic acid (AA), total phenolics (TP), flavonoids (TF), tannin and antioxidant activity (AOX). Results indicate that fenugreek is a good source of flavonoid and total phenolics ranging from 2.74to 12.03 mg/g and 22.59 to 62.59 mg/g, respectively. Diosgenin content ranged from, 6.12 to 10.98 mg/g respectively. One way ANOVA analysis was performed to find the functional attributes which would account for most of the variance.…

    • 1823 Words
    • 8 Pages
    Good Essays
  • Satisfactory Essays

    Effect of sayote

    • 470 Words
    • 2 Pages

    Analysis of variance (ANOVA) at 0.5 level of significance did not reveal differences in the vase life days of flowers after treatment with natural extracts from Garlic, Oregano and Guava. A modification of procedures for extracting natural compounds from guava is recommended. The researchers also recommended the combinations of extract for more favorable results. This research has varied application in the field of Horticulture and food production.…

    • 470 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    Food Chemistry

    • 3529 Words
    • 15 Pages

    Antioxidant activity and hepatoprotective potential of Phyllanthus niruri, a widely used medicinal plant, were investigated. Methanolic and aqueous extract of leaves and fruits of P. niruri showed inhibition of membrane lipid peroxidation (LPO), scavenging of…

    • 3529 Words
    • 15 Pages
    Better Essays