Full Length Research Paper
Screening of chemical composition of essential oil, mineral elements and antioxidant activity in Pulicaria Undulata (L.) C. A. Mey from Iran Mehdi Ravandeh1, Jafar Valizadeh2*, Meissam Noroozifar1 and Mozhgan Khorasani-Motlagh1 1
Department of Chemistry, University of Sistan and Baluchestan, Zahedan, P. O. Box 98155-987, Iran. 2 Department of Biology, University of Sistan and Baluchestan, Zahedan, P. O. Box 98155-987, Iran. Accepted 17 March, 2011
Pulicaria undulata (L.) C.A. Mey is a medicinal plant used to treat inflammation. It is also an insect repellent and herbal tea. In this study, concentration of macro minerals (that is, Ca, K and Mg) and micro minerals (major and trace elements) (that is, Fe, Cu, Na and Mn) and P, were estimated in Pulicaria undulata from the Sistan and Baluchestan province. Sample preparation using microwave digestion was used for mineralization of this plant. Atomic absorption spectrophotometry (AAS) and UV-Visible spectrophotometry were used to quantify metal levels and Phosphorus, respectively. Essential oil from aerial parts of P. undulata was obtained by hydrodistillation. The chemical constituents of essential oil of aerial parts were analyzed by GC-FID and GC/MS techniques. The main components identified in the oil were 4-Terpineole (20.12%), alpha terpinene (4.02%), gamma terpinene (7.00%), cis-sabinene hydrate (8.29%), Linalool (5.60%), 1S-cis-calamenene (13.37%) and Junipene (8.66%). In addition, antioxidant activity of ethanolic extract was evaluated by 1,1-diphenyl-2picrylhydrazyl (DPPH) radical scavenging method. Key words: Macro and micro minerals, microwave digestion, Pulicaria undulata, antioxidant activity. INTRODUCTION Essential oils are volatile, natural, complex compounds characterized by a strong odour and are formed by aromatic plants as secondary metabolites. In nature essential oils play an important role in the protection of the plants as antibactrials, antivirals, antifungals and insecticides. They are usually obtained by steam or hydro- distillation. Known for their antiseptic, that is, bactericidal, virucidal, fungicidal, medicinal properties and their fragrance (Bakkali et al., 2008). There is our day, an increasing interest in the measurement and use of plant antioxidants for scientific research as well as industrial purposes. This is mainly due to their strong biological activity, exceeding those of many synthetic antioxidants which have possible activity as promoters of carcinogenesis (Suhaj, 2006). Therefore, the need exits for safe, economic, powerful and natural antioxidants to replace these synthetic ones (Tadhani et al., 2007). Scientists could qualitatively detect small amounts of several minerals elements in living organisms. The trace elements found in living organisms may be essential, that is, indispensable for growth and health or they may be nonessential, fortuitious reminders of our geochemical origins or indicators of environmental exposure (Ozcan, 2004). Human as well as animal, studies originally showed that optimal intakes of elements such as calcium, sodium, potassium, manganese, magnesium and copper could reduce individual risk factors including those related to cardiovascular disease (Mertz, 1982; SanchezCastillo et al., 1998). Pulicaria undulate (L.) C. A. Mey.
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J. Med. Plant. Res.
[syn. Pulicaria crispa (forssk.) Olive.Francoeuria crispa (forssk.) Cass] is an annual herb or sometimes a perennial sub shrub belonging to the family Asteraceae producing small bright yellow flowers. This species is distributed in Iran, Saudi Arabia, Kuwait, Iraq, Egypt, Afghanistan, Pakistan, India and parts of North and West tropical...