M. Officinalis Lab Report
Materials and methods Plant material
Aerial parts of wild-growing M. officinalis were collected during April from Aum-Romanna (Jordan) by one of us (EYQ). The plant material was identified and authenticated taxonomically at the Hashemite University herbarium. A voucher specimen was deposited under the number HU-437 at the Hashemite University herbarium, Zarka, Jordan, for future reference.
Determination of essential oil composition
Samples of dried aerial parts (300g each sample) of M. officinalis were hydrodistilled for 4 h in a modified Clevenger-type apparatus to obtain the volatile constituents. The essential oil of M. officinalis was analysed by GC on a Trace GC ULTRA with FID detector gas chromatograph equipped with a column (30 m …show more content…
The experiment was carried out in two phases; in the first phase, geometric doses of the MOEO (10, 100 and 1000 mg/kg) were administered i.p. to three groups of mice, and the control group received normal saline (10 ml/kg, i.p.). In the second phase, other doses of the MOEO (1600, 2900 and 5000 mg/kg) were administered. Signs of toxicity and mortality within 24 h were noted. The LD50 was then calculated based on the pattern of death observed in the second phase using the Probit-log analysis from the graph of percent mortality against log dose of the …show more content…
Sixty min later, each rat received 50 μL of formalin (5%) subcutaneously into the dorsal surface of the right hind paw using a microsyringe with a 27 gauge needle. Immediately after formalin injection, animals were placed individually in acrylic observation chambers (320 cm2 × 40 cm). Mirrors were arranged at angles to allow clear observation of the paws of the animals. Licking of the injected paw was defined as the nociceptive response. The total time of the response was measured during the periods of 0–5 min (early phase) and 15–40 min (late phase). The inhibition of licking (in %) was calculated by the formula: (A-B/A) × 100, where A is the time of licking before treatment; B is the time of licking after
Aerial parts of wild-growing M. officinalis were collected during April from Aum-Romanna (Jordan) by one of us (EYQ). The plant material was identified and authenticated taxonomically at the Hashemite University herbarium. A voucher specimen was deposited under the number HU-437 at the Hashemite University herbarium, Zarka, Jordan, for future reference.
Determination of essential oil composition
Samples of dried aerial parts (300g each sample) of M. officinalis were hydrodistilled for 4 h in a modified Clevenger-type apparatus to obtain the volatile constituents. The essential oil of M. officinalis was analysed by GC on a Trace GC ULTRA with FID detector gas chromatograph equipped with a column (30 m …show more content…
The experiment was carried out in two phases; in the first phase, geometric doses of the MOEO (10, 100 and 1000 mg/kg) were administered i.p. to three groups of mice, and the control group received normal saline (10 ml/kg, i.p.). In the second phase, other doses of the MOEO (1600, 2900 and 5000 mg/kg) were administered. Signs of toxicity and mortality within 24 h were noted. The LD50 was then calculated based on the pattern of death observed in the second phase using the Probit-log analysis from the graph of percent mortality against log dose of the …show more content…
Sixty min later, each rat received 50 μL of formalin (5%) subcutaneously into the dorsal surface of the right hind paw using a microsyringe with a 27 gauge needle. Immediately after formalin injection, animals were placed individually in acrylic observation chambers (320 cm2 × 40 cm). Mirrors were arranged at angles to allow clear observation of the paws of the animals. Licking of the injected paw was defined as the nociceptive response. The total time of the response was measured during the periods of 0–5 min (early phase) and 15–40 min (late phase). The inhibition of licking (in %) was calculated by the formula: (A-B/A) × 100, where A is the time of licking before treatment; B is the time of licking after