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Science Investigatory Project
Saccharification of Pineapple Ananas comosus peelings through Dilute Acid Hydrolysis

Rhoy M. Capul Ma. Michelle Carmel O. Aquino Alia Jenine S. Caceres

San Pablo City National High School San Pablo City

Mrs. Glory M. Serrano Research Adviser

A Science Investigatory Project in fulfillment of Research project S.Y. 2010-2011 Abstract
The pineapple is one of the leading products in the Philippines, as it thrives in tropical climates. However, the pineapple peelings have low commercial value and are therefore merely thrown away, contributing to the Philippines waste problem.
This study entitled “Saccharification of Pineapple Ananas comosus peelings through Dilute Acid Hydrolysis” was conducted to know the concentration of sugar content which can be used for other purposes like bioethanol. Three samples were used, each sample was composed of two trials; S1T1 (60 degree Celsius for 30 min.), S1T2 (60 degree Celsius for 60 min.), S2T1 (70 degree Celsius for 30 min.), S2T2 (70 degree Celsius for 60 min.), S3T1 (80 degree Celsius for 30 min.), S3T2 (80 degree Celsius for 60 min). The phenol- sulfuric acid method was used to determine the concentration of sugar content present in the substrate.
The test revealed that the dilute acid hydrolysis is an effective way and can saccharify pineapple peelings based on the standard curve. However, the temperature and time was found out that they both have no significant difference in yielding greater concentration of sugar as what as the statistical analysis revealed using Kruskal-Wallis test and Mann Whitney U-test.

INTRODUCTION
Background of the study
Pineapple (Ananas comosus) is the common name for a tropical plant and its edible fruit which are coalesced berries. Pineapples are the only bromeliad fruit in widespread cultivation. It can be grown as an ornamental, especially from the leafy tops. Some sources say that the plant will flower after about 24 months &

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