Respiratory Quotient

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Measuring the Respiratory Quotient

Table 1 Representing the raw data of the Gas Pressure(kPa) in an enclosed environment during respiration of mung beans and sunflower beans with and without soda lime.

Time in seconds(+/-1)| Mung Beans(+/-0.01kPa| Mung Beans with Soda Lime (+/-0.01kPa)| Sunflower Beans(+/-0.01kPa| Sunflower with Soda lime(+/-0.01kPa| 0| 100.97| 103.29| 100.97| 100.93|

50| 100.49| 102.85| 100.45| 100.48|
100| 100.07| 102.51| 99.89| 100.07|
150| 99.77| 102.24| 99.33| 99.71|
200| 99.45| 102.01| 98.94| 99.34|
250| 99.22| 101.82| 98.59| 99.06|
o| 99.04| 101.93| 98.35| 98.76|

Graph1 Representing the Gas Pressure recorded in an enclosed environment during respiration in mu ng beans and sunflower beans with and without soda lime.

Graph 2 : Representing the gradient of Gas Pressure released in an enclosed environment during respiration in mung beans with and without soda lime

Graph 3 : Representing the gradient of Gas Pressure released in an enclosed environment during respiration in sunflower beans with and without soda lime

RQ calculation of Glucose:
The Respiratory Quotient (RQ) is calculated from the formula, RQ = CO2 eliminated / O2 consumed
The equation for the respiration of Glucose( C6H12O6 )

C6H12O6 + 6O2 6CO2 + 6H20

Therefore RQ = 6 molecules of CO2 eliminated / 6 molecules of O2 consumed = 6/6
RQ for glucose = 1
Calculating the RQ of mung beans :
From the above formula of RQ, we can calculate the amount Oxygen consumed, from gradient of the graph of mung beans with soda lime. Soda lime is known to absorb carbon dioxide, therefore the carbon dioxide that was released during the respiration process would be absorbed by the soda lime, leaving the oxygen hence the value of oxygen consumed can be determined and so to get the numerical value of the oxygen consumed,the gradient function of the Logger pro was used as shown in the...
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