Pseudomonas Lab
Pseudomonas aeruginosa
Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had, was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane, periplasmic space, peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any colony pigment. It appeared as a circular, raised, undulated bacterium. The arrangement of Pseudomonas aeruginosa is rod shaped. Multiple biochemical tests can be performed to determine if gram positive or negative. See Table below:
Oxidase …show more content…
Problems that may arise with testing could be that the bacteria being tested could be specimen contamination or the specimen may require multiple tests to determine which bacteria that it is resulting in higher cost and greater contamination probability. Pseudomonas aeruginosa is a member of the Gamma Proteobacteria class of bacteria. It is a gram negative bacteria, aerobic rod belonging to the bacteria family Pseudomonadaceae. It is a free living bacterium commonly found in soil and water. Pseudomonas aeruginosa is an opportunistic pathogen, meaning that it exploits some break in the host defenses to initiate an infection. The bacterium almost never infects uncompromised tissues, yet there is hardly any tissue that it cannot infect if the tissue defenses are compromised in some manner. Burn victims and people with puncture wounds may get dangerous pseudomonas infections of the blood, bone, or urinary tract. The bacteria can also get into the body through IV needles or catheters. Motility occurs via flagella, and retractile pili. Adhesins occur by way of pili and alginate slime. …show more content…
This can happen if you aren 't careful about keeping your contact lenses and equipment sterile. It causes urinary tract infections in people with Foley catheters, respiratory system infections in people with mechanical ventilation, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections and a variety of systemic infections, particularly in patients with severe burns and in cancer and AIDS patients who are immunosuppressed.(Giamarellou) Pseudomonas aeruginosa is naturally resistant to a large range of antibiotics. This makes treatment for such bacteria very difficult at times, especially with certain patient allergies. In hospitals, where the most serious infections occur, Pseudomonas can be spread on the hands of healthcare workers or by equipment that gets contaminated and is not properly cleaned, hence why infection control in-services are vital to patient
Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had, was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane, periplasmic space, peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any colony pigment. It appeared as a circular, raised, undulated bacterium. The arrangement of Pseudomonas aeruginosa is rod shaped. Multiple biochemical tests can be performed to determine if gram positive or negative. See Table below:
Oxidase …show more content…
Problems that may arise with testing could be that the bacteria being tested could be specimen contamination or the specimen may require multiple tests to determine which bacteria that it is resulting in higher cost and greater contamination probability. Pseudomonas aeruginosa is a member of the Gamma Proteobacteria class of bacteria. It is a gram negative bacteria, aerobic rod belonging to the bacteria family Pseudomonadaceae. It is a free living bacterium commonly found in soil and water. Pseudomonas aeruginosa is an opportunistic pathogen, meaning that it exploits some break in the host defenses to initiate an infection. The bacterium almost never infects uncompromised tissues, yet there is hardly any tissue that it cannot infect if the tissue defenses are compromised in some manner. Burn victims and people with puncture wounds may get dangerous pseudomonas infections of the blood, bone, or urinary tract. The bacteria can also get into the body through IV needles or catheters. Motility occurs via flagella, and retractile pili. Adhesins occur by way of pili and alginate slime. …show more content…
This can happen if you aren 't careful about keeping your contact lenses and equipment sterile. It causes urinary tract infections in people with Foley catheters, respiratory system infections in people with mechanical ventilation, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections and a variety of systemic infections, particularly in patients with severe burns and in cancer and AIDS patients who are immunosuppressed.(Giamarellou) Pseudomonas aeruginosa is naturally resistant to a large range of antibiotics. This makes treatment for such bacteria very difficult at times, especially with certain patient allergies. In hospitals, where the most serious infections occur, Pseudomonas can be spread on the hands of healthcare workers or by equipment that gets contaminated and is not properly cleaned, hence why infection control in-services are vital to patient