Potato Enzyme Experiment

Introduction: Enzymes are a substance produced by a living organism that acts as a catalyst to activate a specific reaction. The purpose of this experiment was to figure out if the temperature of the reaction would rise, will the absorption rise as well. Reactions use energy, If there is energy than heat occurs. The Hypothesis that was figured out was, If the temperature rises, then the absorption will also go up. The Independent variable that was tested was temperature. The dependent variable that was being tested was color and absorption. The data was calculated by a spectrophotometer. The control group of this experiment was room temperature. The constant variables were, the amount of distalled water, catechol substrate, and potato extract in each tube was the same. (2ml) The size of the test tubes were the same also.
Next you will need to acquire a wax pencil and label the test tubes 1-3. You will then need to measure out 2ml of distilled water and 2ml of catechol substrate. After everything is measured out and put into the cuvettes, stir the liquid around a little. After this step, insert 2ml, of potato extract and put the laboratory film on top of the cuvettes. Then, quickly transport the cuvettes to their appropriate locations. (Ice bath, hot plate,and leave one alone at room temp.) After a little time has passed remove them and use the task wipes to remove any debri on the exterior of the cuvettes. Record the color changes. Zero the spectrophotometer, then place each sample in the spectrophotometer. Record the number that displays on the screen. After all data has been recorded, then you must clean the test tubes. Wipe of the wax with the task whips. After that step was complete, carefully wash the cuvettes. Use a test tube brush if necessary. Use soap, than rinse. Store the cuvettes upside down for them to