Photosynthetic Pigments

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BIOLOGY LAB REPORT
NAME :princess cucumber
TOPIC : 3.8 & 8.2 : PHOTOSYNTHETIC PIGMENTS
RESEARCH QUESTION

INTRODUCTION
The photosynthetic pigments are responsible for absorbing and trapping light energy the major pigments of photosynthesis are the chlorophylls. The two chlorophylls found in green plants are chlorophyll a and chlorophyll b. other pigments include carotenoids and phycobilins, sometimes referred to as accessory pigments. Carotenoids occur in all photosynthetic organisms, while phycobilins occur in the red algae and cyanobacteria.

HYPOTHESIS
VARIABLES
Variables | Methods to handle|
Manipulated variable :| |
Responding variable :| |
Constant variable: 1. Volume of pure acetone 2. Molarity of | 1. | Table 1 shows the variables in this experiment
APPARATUS & MATERIALS
Apparatus/ materials| Quantity |
Large boiling tube| 1|
Large test tube rack| 1|
Cork stopper with pin hook| 1|
Strip of filter paper (Goodman)| 1|
Capillary tube| 1|
Fresh spinach leaves| 10g|
Fresh solnostemon hybrid leaves| 10g|
Chromatography solvent (9:1 petroleum ether-acetone)| 3mL| Measuring cylinder (20mL)| 1|
Strainer | 1|
Acetone (100%)| 40mL|
Calcium carbonate| As much as needed|
Pestle and mortar| 1|
Electronic balance| 1|
Scissors| 1|
Ruler | 1|
Measuring cylinder (15mL)| 1|
Beaker (50mL)| 1|
Table 2 shows the apparatus and materials used in this experiment METHOD
Preparation of spinach pigment extract:
1. 10g of fresh spinach leaves ( without the veins ) is taken and is placed into a mortar. 2. A pinch of calcium carbonate is added into the mortar. The pestle is used to grind the leaves and calcium carbonate until it forms a paste 3. 20mL of pure acetone is added and the mixture is stirred until a dark green solution is formed 4. The concentrated pigment extract is separated using a strainer Preparation of chromatogram:

5. A piece of filter...
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