Paper Chromatography Of Blueberries
David Noviski
CHM 244 B
Dr. Bauman
Isuru Wanigasooriyage Rachel Oliverio
11/1/16
Analysis of Anthocyanins and Anthocyanidins in Blackberries, Raspberries, and Blueberries
Abstract
The purpose of this experiment was to isolate anthocyanins, decompose them into their components (anthocyanidins), and to analyze the anthocyanins, and their corresponding anthocyanidins using paper chromatography, and spectrophotometric analysis procedures. Based on experimental data the blueberries contained the most amount of anthocyanins at 1.92 x 10-6 g, followed by blackberries at 1.19 x 10-6 g, finally followed by raspberries at 5.84 x 10-7 g. The paper chromatography data also supports the idea that blueberries contained the most anthocyanidins, since it contained the most streaks on the chromatography paper. The main conclusion that can be drawn from this experiment is that blueberries contain the most anthocyanins per gram compared to raspberries and blackberries.
Introduction
The purpose of this experiment was to isolate anthocyanins, decompose them into their components (anthocyanidins), and to analyze the anthocyanins, and their corresponding anthocyanidins using paper chromatography, and spectrophotometric analysis procedures. Anthocyanins, “Are responsible for most of the red, purple, and blue colors exhibited by flowers, …show more content…
A spectrophotometer can be used to analyze a solution containing anthocyanidins by locating the maximum wavelength and its corresponding absorbance value. These values can help identify which anthocyanidins are in the solution, as well as how much anthocyanidin, and by extension how much anthocyanin was present in the original
CHM 244 B
Dr. Bauman
Isuru Wanigasooriyage Rachel Oliverio
11/1/16
Analysis of Anthocyanins and Anthocyanidins in Blackberries, Raspberries, and Blueberries
Abstract
The purpose of this experiment was to isolate anthocyanins, decompose them into their components (anthocyanidins), and to analyze the anthocyanins, and their corresponding anthocyanidins using paper chromatography, and spectrophotometric analysis procedures. Based on experimental data the blueberries contained the most amount of anthocyanins at 1.92 x 10-6 g, followed by blackberries at 1.19 x 10-6 g, finally followed by raspberries at 5.84 x 10-7 g. The paper chromatography data also supports the idea that blueberries contained the most anthocyanidins, since it contained the most streaks on the chromatography paper. The main conclusion that can be drawn from this experiment is that blueberries contain the most anthocyanins per gram compared to raspberries and blackberries.
Introduction
The purpose of this experiment was to isolate anthocyanins, decompose them into their components (anthocyanidins), and to analyze the anthocyanins, and their corresponding anthocyanidins using paper chromatography, and spectrophotometric analysis procedures. Anthocyanins, “Are responsible for most of the red, purple, and blue colors exhibited by flowers, …show more content…
A spectrophotometer can be used to analyze a solution containing anthocyanidins by locating the maximum wavelength and its corresponding absorbance value. These values can help identify which anthocyanidins are in the solution, as well as how much anthocyanidin, and by extension how much anthocyanin was present in the original