April 22, 2013
MHC and Organ Transplant Paper
There are a few routes that sensitization to non-self human leukocyte antigens (HLA) occurs (Fuggle & Martin, 2008). This article shows that 23% of renal patients that are awaiting transplantation are sensitized. More females than males have the HLA antigen. It also shows that first time transplants have less HLA antibodies than those that have had transplants in the past. Several approaches to transplant sensitized patients are presently used or have been used in the past. It is important to understand the different techniques but knowing why is just as important. One can say that organ sharing schemes is one important approach. This process can use the cross-match-negative organs with a larger donor pool in the National Transplant Database. For example in the UK, “the identification of suitable donors for sensitized patients has been facilitated by a requirement for accurate definition of a patient’s antibody profile,” (Fuggle & Martin, 2008, para. 3). There are different techniques that both histocompatibility and Immunogenetics labs try for alloantibody detection and specific definition for those patients that are waiting those transplants. All the strategies that are utilized in defining the HLA antigens make them unacceptable to prospective donors. These unacceptable antigens are registered on the National Transplant Database and these patients that fall under these criteria will not be eligible for those transplants. “Thankfully there a range of techniques that are available for the detection and definition of HLA specific antibodies and to interpret the results from the various assays in is important to understand the different principles underlying the assays as well as their relative strengths and weakness,” (Fuggle & Martin, 2008, para. 5). Cell Based Assay
According to Fuggle & Martin, (2008) Compliment Dependent Cytotoxicity (CDC) assay...