Metabolic Rate

Topics: Carbon dioxide, Metabolism, Beetle Pages: 3 (887 words) Published: March 8, 2013
Resting metabolic rate can vary with age independently from body mass changes in the Colorado potato beetle, Leptinotarsa decemlineata. Abstract
A study was done by Saija Pironine, leena Lindstrom, and Anne Lyytinen in the Journal of Insect Physiology, to investigate the age dependence of resting metabolic rate in Colorado potato beetle (Leptinotarsadecemlineata) from adult emergence until age 10 days. In their hypothesis they expected to see a bell shaped pattern in metabolic rate with adult age. Metabolic rate will increases from emergence day to 2-day old and then decreases again. They noticed that age had a significant effect on metabolic rate. During the first 2 days of adult life a twofold increase in metabolic rate occurred which then decreased with age relatively slow. In their study they used the flow-through respirometry method to measure carbon dioxide production which is the same method we used in our Bio 120 lab to determine how metabolic rate is affected by body mass. Introduction

Many studies have been done on metabolic rate and how it is affected by mass and temperature, but not many studies have been done on MR and how age affects it. Age has a pronounced effect on metabolic rate. Metabolic rate is assumed to decrease with adult age due to deterioration of the physiological condition associated with ageing, but metabolic rate can is also assumed to increase with adult age due to higher energetic demands. Methods

In this study production of CO2 was used to measure metabolic rate. Total of 108 beetles were measure in this experiment from age 0 to 10 days. The beetles were starved for 4 hours before measurement to avoid CO2 emission resulting from assimilation of food. 30 beetles (group1) were measure at ages 0, 4, and 8 days. 28 beetles (group 2) were measure at ages 1, 5, and 9 days. 25 beetles (group 3) were measured at ages 2, 6, and 10 days. 25 beetles (group 4) were measured at ages 3 and 2 days. Individuals were divided evenly into...
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