Bio 1000 Manometric System Lab
“Comparing Average Rates of Respiration in Mealworms in Light and Dark Environments in a Manometric System”
Mohammad Rafi
213552179
BIOL1000 LAB REPORT
Oct 28, 2014
Hypothesis
The biological hypothesis for this lab experiment was that the rate of respiration in mealworms would decrease in mealworms that were exposed to a dark environment compared to those mealworms that were exposed to a light environment. This hypothesis was based on the fact that oxygen levels tend to decrease in light deficient environments. Due to limited oxygen, citrate concentrations found in the citric acid cycle are high and affects the activity of the enzyme phosphofructokinase, which is found in the early stages of glycolysis and catalyses the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate, by slowing down its function and therefore slowing down cellular respiration (Russell, Hertz, McMillan, Fenton, Addy, Maxwell, Haffie, MIlsom 2014). The null hypothesis was that the rate of respiration in mealworms would not change if they are in a dark environment from those in light environment. The alternate hypothesis was that there will be a decrease in the rate of respiration in mealworms from the dark environment.
Results
The rate of respiration in mealworms from the twelve experimental tubes in the light treatment ranged from 0.0489 mL O2/g/hr to 1.1611 mL O2/g/hr. The average rate of respiration was determined to be 0.4034 ± 0.1886 mL O2/g/hr. The rate of respiration in mealworms from the six control tubes of light treatment ranged from 0 mL O2/g/hr to 0.0859 mL O2/g/hr. The average rate of respiration of them was determined to be 0.0208 ± 0.0068 mL O2/g/hr. The CI bars of the experimental tubes and the control tubes in this treatment did not overlap, so they were determined to be significantly different from each other. Meanwhile, in the mealworms from dark treatment, their rate of respiration in the twelve experimental tubes ranged from 0.0417 mL
Mohammad Rafi
213552179
BIOL1000 LAB REPORT
Oct 28, 2014
Hypothesis
The biological hypothesis for this lab experiment was that the rate of respiration in mealworms would decrease in mealworms that were exposed to a dark environment compared to those mealworms that were exposed to a light environment. This hypothesis was based on the fact that oxygen levels tend to decrease in light deficient environments. Due to limited oxygen, citrate concentrations found in the citric acid cycle are high and affects the activity of the enzyme phosphofructokinase, which is found in the early stages of glycolysis and catalyses the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate, by slowing down its function and therefore slowing down cellular respiration (Russell, Hertz, McMillan, Fenton, Addy, Maxwell, Haffie, MIlsom 2014). The null hypothesis was that the rate of respiration in mealworms would not change if they are in a dark environment from those in light environment. The alternate hypothesis was that there will be a decrease in the rate of respiration in mealworms from the dark environment.
Results
The rate of respiration in mealworms from the twelve experimental tubes in the light treatment ranged from 0.0489 mL O2/g/hr to 1.1611 mL O2/g/hr. The average rate of respiration was determined to be 0.4034 ± 0.1886 mL O2/g/hr. The rate of respiration in mealworms from the six control tubes of light treatment ranged from 0 mL O2/g/hr to 0.0859 mL O2/g/hr. The average rate of respiration of them was determined to be 0.0208 ± 0.0068 mL O2/g/hr. The CI bars of the experimental tubes and the control tubes in this treatment did not overlap, so they were determined to be significantly different from each other. Meanwhile, in the mealworms from dark treatment, their rate of respiration in the twelve experimental tubes ranged from 0.0417 mL
References: Russell P, Hertz PE, McMillan B, Fenton B, Addy H, Maxwell D, Haffie T, Milsom B. 2014. Biology: Exploring the Diversity of Life. York University, Custom Edition