Lab 1: Microscopy and the Metric System
1. List the components of the compound microscope and their function. 2. determine the total magnification given that you are using a compound microscope with the following objectives: 4x, 10x, 40x, and 100x 3. what is meant by the depth of field?
4. what is meant by the field of view?
5. describe the process of making a wet mount.
________________________________________________________________________________ 1. -Focal adjustment; used to adjust height of stand to adjust closer too or farther from slide. -eyepiece; used to look into to see what is on the slide closer -arm; hold when carrying, also used to
-objective lenses; must use smallest objective lens, they have different levels of magnification. -stand; used to hold up the microscope.
-light source; needs to be turned on to be able to see.
-base; hold when carrying, also used for standing up microscope. 2. 4x=40x , 10x=100x , 40x=400x , 100x=1000x
3. Depth of field is the distance between the nearest and the furthest objects that give an image its focus in a camera. 4. Field of view is the area that is visible.
5. Mix sample to make sure it is properly suspended. Take dropper to pick up sample material. Put a small amount on the glass slide. Take a cover glass by the corners with tweezers to not get any finger prints. Put cover from corner down to avoid air bubbles. If too much water use a tissue or filter paper to suck up remaining water. Part B:
1. Linear measurements: measure the width of your textbook in cm and then convert to mm. 2. Conversions: convert 100 grams to mg and then ug
3. Weight measurements: using your scale, record the mass of an object in grams and include the name of the object you have measured. Once you have recorded your measurements in grams, please convert the measurement to mg and then ug. 4. Volume measurements: define meniscus and describe how you would read the volume of a liquid in a gradulated cylinder....
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