Inheritance Patterns - Genetics - Senior Biology

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Trinity Lutheran College

2012
Inheritance Patterns
Extended Experimental Investigation
Scott Zimmerman

The Abstract:

Contents
The Task5
Timeline5
Experimental Design6
Introduction9
Preliminary Hypothesis Justification9
Planning10
Gantt chart10
Variables11
Dependant - Genotypes11
Phenotypes12
Independent12
Inheritance Patterns12
Constant13
Sunlight13
Water14
Soil15
Families & Generations16
Peas16
Barley17
Materials18
Risk Assessment Forum19
Method21
Results: - Barley22
Peas23
Year 12 Biology Cohort Collective Result25
Collective Graphs - Peas26
Barley27
Spectrometer – Peas28
Barley29
Analysis, Discussion and Interpretation of Data30
Conclusion32
Evaluation32
Appendices33
133
233
Glossary34
Bibliography35

The Task

We will be provided with 30 Barley and 15 Pea seeds, some of which have mutations causing defective chlorophyll. We need to investigate the effect of the mutation on the plants and then determine the type of inheritance pattern shown in each case. The seeds need to be germinated and then grown for a length of time sufficient to determine and measure the effect of the mutations on the plant phenotypes. By measuring various characteristics of the growth rates of the germinated seeds, as well as performing experiments to determine the relative amounts of chlorophyll present, we should be able to scientifically describe the effects of the different mutations. Using the total number of the different phenotypes from our class results, we need to determine the type of inheritance pattern that the mutations show.

Timeline
Issue date: March 28th
Seedling Germination: April 6th
Draft of report submission: May 14th
Submission of topic: May 21st
Final report, and logbook submitted (up to 2500 words)

Experimental Design

Aim:State your hypothesis from the Hypothesis W/S (“If..then..”statement) Each plant with the Green Phenotype and the GxG / Gxg Genotype (where the g: yellow/white is recessive) will grow much more effectively than those with a White or Yellow Phenotype with the Genotypes of gxg. Design: Describe how you could test your hypothesis

By measuring the plant collective daily the hypothesis will be proven through continual comparison of each species. At the end of the measuring period each species and Phenotype will be weighed to determine overall biological mass and which point the overall growth rate for each phenotype will be given. Materials: List the equipment you will need

Part A:
* Water
* Measuring Utensils
* Seed Planter
* Seeds
* Ruler
* Soil
Part B:
* Test tubes
* Beakers
* Hot Plate
* Scales
* Cutting Utensils
* Light absorption meter
* Tongs
* Boiling Chips

Method: What will you change (called the independent variable) and what will you measure? (dependant variable) What will you keep the same (the controlled variables) and how will you accomplish this? We will be measuring the growth rate of the collective for each species and phenotype. The independent variable will be the phenotypes within each species (ie White/Green & Dark Green, Green & Yellow). I plan to keep the amount of water at a constant by watering each individual with 5mL of water daily and preventing rain from affecting them. Sunlight will also be controlled by the placement of the seed planters, had the sun gone out due to rain the plants would have been placed under UV filters for the interim. The soil was kept at a constant due to the use of similar soil for each individual seedling.

Part A:
* Allow the seeds to germinate before placing them into the soil filled seed planter * Water the seedlings daily, assure that enough sun is available for your seedlings * Take measurements of all seedlings as soon as the first shoot appears out of one of the seed boxes. * Repeat Steps 2 & 3...
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