The main objective of this lab was to observe the activity of enzyme peroxidase in real time under different experimental conditions. To see how peroxidase reacts with its substrate guaiacol under different conditions. We measure the amount of substances per test tube and then combine all of them together. Each test tube contains a measured amount of any listed substances including H₂O. Each measured amount of peroxidase along with its substrate guaiacol, and other listed substances will show how actively the enzyme oxidizes the substrate. To measure the…
2. Using forceps and scissors cut a small piece of liver and add it to the test tube. Push it into the hydrogen peroxide with a stirring rod. Observe the bubbles.…
4. Different concentrations of hydrogen peroxide solution were added to each test tube using a pipette. (5mL of 6%, 3% and 1.5%)…
Test tube was hot after reaction; exothermic reaction took place; sodium was malleable; when sodium was added to diH2O it popped and fizzled; solution turned pink when phenolphthalein was added.…
B. Using a micropipette, place a few drops of hydrogen peroxide (H2O2) on top of the liver.…
The experiment performed in the second part of the lab was heating 4 different chemical substances (s) and observing the changes made to them. Also determining if a chemical change had occurred. One test was performed with the substances in the tubes and another with the chemicals outside of the tubes and over the open flame.…
10) Fill test tube #5 with sand and a piece of liver. Then add 1mL of Hydrogen Peroxide.…
Procedure: In this experiment I used a 96-well plate to hold each of the chemical reactions that occurred during this lab. Using a pipet, I added two drops of one chemical with two drops of another chemical and observed the effects of each reaction.…
1. Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…
Procedure: In this experiment, various chemicals were mixed together, to determine a reaction. Using two drops from chemical 1 and two drops of chemical two, unless otherwise stated, then recording the type of physical reaction or color changes that occurred.…
Preparation Step 1: Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…
We start to fill test tube 1 4drops of HCI (acid), test tube 2 dilute HCI 1 drop and 3ml water, test tube 3 add 4 drops of NaOH (Base) , test tube 4 dilute NaOH 1 drop and 3ml water, test tube 5 add 3 drops of water (neutral). We filled each tube with the “liver solution to see if there is any reaction in the different test tubes.…
After mixing water, sodium hydroxide and a drop of phenolphthalein, the water changed its color from transparent to a pink. As more phenolphthalein was add the more dark the pink got. Since the change in color is one way of detecting chemical reaction, the conclusion was that in this specific experience chemical reaction happened.…
Hydrogen Peroxide is a widely used chemical that has many different uses, many different safety hazards, and different dangerous hazards. “Hydrogen…
The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level).…