Scientific Methodology & Enzyme Activity Essay Example

Laboratory 2: Scientific Methodology & Enzyme Activity

Objective: The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level).

Hypothesis:
Part a:
If different amounts of enzyme solution are added to the hydrogen peroxide, then the highest amount of enzymes will have the greatest reaction rate because enzymes catalyze reactions, meaning more oxygen will be produced quicker.

Part b:
If different temperatures are used to catalyze enzyme activity, then the lowest temperature would produce the quickest reaction rate because enzymes can become denaturized at higher temperatures

Part c:
If different amounts of pH are used to catalyze the reaction, then the concentration with pH 7 will have the quickest reaction, because enzymes work best in neutral conditions such as conditions that are neither acidic nor alkaline.
Procedures:
Part a:
Connect Gas Sensor to Lab quest
Label 4 test tubes and fill each with 5mL of 3.0% hydrogen peroxide and 5mL of water
Add 5 drops of enzyme solution to test tube 1
Pour contents of tube 1 into Nalgene bottle, place O2 Gas Sensor in.
Perform linear regression to calculate rate of reaction.
Fine rate of enzyme activity for test tubes 2,3,4
Add 10 drops of solution test tube 2. Repeat steps 6-8
Add 20 drops of enzyme solution to test tube 3. Repeat 6-8
Repeat steps 6-8 without any enzyme drops.

Part b:
Obtain 5 different test tubes. Fill each test tube with 5 mL of 3.0% hydrogen peroxide and 5 mL of water.
Place each tube in each of the following water baths for 10 mins
0 degrees C: 400 mL beaker filled with ice water
23 degrees C: No bath
37 degrees C: Place in bath
70 degrees C: Place in bath
100 degrees C: Obtain hotplate and heat water
Add 10 drops of enzyme solution to all test tubes

Part c:
Place three clean test tubes in a rack and