Bios 221
April 4, 2012
Drosophila
Introduction
The fruit fly or Drosophila melanogaster is ideal for classroom experiments. They are inexpensive, easy to nap, breed, as well as observe. It was very important to be able to tell the male and female flies apart from one another. The males are known to be usually smaller in size than the female flies and have bristles on their forelegs while the females lack this appearance. Also the males have a black or dark round end whereas the females have striped pointy ends. The Drosophila flies are small have dark red eyes and have a yellow-brown body. These flies are able to mutate within approximately ten to fourteen days at twenty-five degrees Celsius. The Drosophila has a …show more content…
This lab was the study of monohybrid as well as dihybrid crosses. A monohybrid cross is the study of a certain trait whereas a dihybrid cross is the study of the mating experiment between two organisms that are identically hybrid for two traits. ( Reference 1 ) This lab was done to determine the genetic mutations after each generation and to observe the ratios. As each generation of fruit flies came to existence, traits would either be different from each fly or certain traits would disappear from existence. The expected ratio for a monohybrid cross was 3:1 and 9:3:3:1 for the dihybrid cross. For the monohybrid cross, eye color was observed to be wildtype, dark red, or white eyed, which was x-linked. (Reference 2) As for the dihybrid cross, both wing shape as well as eye color was observed. The wings were either straight, wildtype, or shriveled, vestigial, depending on …show more content…
The wand was dipped into the Flynap and then placed into the tube for a few minutes until the flies were asleep. Once the flies were asleep, the wand was removed and the flies were distributed. From this point, the flies were counted and their sex was determined under the microscope. The monohybrid cross was sex-linked, wild x wild eye colored flies. The dihybrid consisted of vestigial winged flies x sepia eye colored. An even amount of males and females were placed in the vial tubes using a teasing needle, one labeled monohybrid and the other dihybrid. Within each tube, there was some culture media for the flies to survive and reproduce for about a week. After giving the fruit flies time to reproduce, they were put to sleep again using the Flynap and then the progeny were taken out to be counted and observed. The results were recorded and the same was done for the following generation,